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1
ELISA
Issues Might Meet in the Process of Using ELISA...
There are some issues that researchers might meet in the process of using ELISA Kits:
1. Positive results in negative control
2. High background across entire plate
3. Low absorbance values
4. High absorbance values for samples and/or positive control - absorbance does not go down as the sample is diluted down the plate
5. Inconsistent absorbance across the plate
6. Color develops slowly
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Elisa Kits, Creative Biomart
