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DNA migrate differently in agarose vs PAGE gel

Aug 15 2011 08:59 PM | pcrman in Electrophoresis

Quotei am currently working a gene in leukemia. The mutant allele has been sequenced to contain 527 base pair. when i run an agarose gel to confirm, it did appear in the position approximately equal to 527 base pair according to the DNA ladder. HOWEVER...

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