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How to convert g into rpm? - Centrifugation - BioWiki Knowledge Base - BioWiki Knowledge Base - BioForum - Protocol Online

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How to convert g into rpm?



The relationship between revolutions per minute (RPM) and relative centrifugal force (xg) is:
g = (1.118 10-5) R S2
where g is the relative centrifugal force, R is the radius of the rotor in centimeters, and S is the speed of the centrifuge in
revolutions per minute.

You can use this for any centrifuge, just measure the radius of the rotor from the center to outer edge.

2 Comments

Are centrifugation at 10000xg (?rpm) and ice incubation essential in colorimetric protease assay of caspase-3?

Or there are some modification as eliminate ice step.
Amr G. Elsayed
Applied Medical Chemistry
Medical Research Institute
Alexandria University
amrbiochemistry@gmail.com
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