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General Lab Techniques

How to convert g into rpm?

May 28 2009 07:05 PM | bioforum in Centrifugation

The relationship between revolutions per minute (RPM) and relative centrifugal force (xg) is: g = (1.118 10-5) R S2 where g is the relative centrifugal force, R is the radius of the rotor in centimeters, and S is the speed of the centrifuge in revolu...

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DNA migrate differently in agarose vs PAGE gel

Aug 15 2011 08:59 PM | pcrman in Electrophoresis

i am currently working a gene in leukemia. The mutant allele has been sequenced to contain 527 base pair. when i run an agarose gel to confirm, it did appear in the position approximately equal to 527 base pair according to the DNA ladder. HOWEVER, whe...

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Useful Dilution Techniques & Calculations

Mar 21 2009 02:33 PM | bioforum in General Lab Techniques

Today was a very bad day at the university... beside that some students answered in the quiz question that if you get blue color in Gram staining that means your bacteria is Gram negative :huh: I noticed most of the students including myself had troub...

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Normalization for RNA or cDNA during two step R...

Apr 20 2009 09:13 AM | bioforum in General Lab Techniques

Curtis 20 April 2009 - 03:13 AM Hello all, I have a two-step SYBR Green RT-PCR kit for RotorGene. After I extract RNA from my samples, I proceed with cDNA synthesis right away without measuring the RNA concentration or wit...

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