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- General Lab Techniques
- Western Blotting
- RNA Methods
- Molecular Cloning
- Cell Culture
- Stem Cells
- General Molecular Biology Methods
- Transcription, epigenetic, epigenomic techniques
- RNAi, miRNA and ncRNA
- Bioinformatics and Biostatistics
- Protein Methods
- FACS Analysis
Cell Culture Archives
jennycharlies Posted 18 August 2011 - 06:55 AM Hi, I am going to seed my cells onto coverslips inside the wells of a 12 well plate and was wondering what is the best way to coat the coverslips? I have 18mm coverslips and need to coat with poly-D-...
I am facing problems in thawing of cells. The procedure which we follow is- we take stock from liq. N2 then though it in water bath at 37 degree. Then we take medium in falcon (5ml) then add thawed cells dropwise into it. We then centrifuge it at 1500...