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Cell Culture

How to coat coverslips before seeding cells?

Aug 18 2011 05:55 AM | jennycharlies in Cell Culture

jennycharlies Posted 18 August 2011 - 06:55 AM Hi, I am going to seed my cells onto coverslips inside the wells of a 12 well plate and was wondering what is the best way to coat the coverslips? I have 18mm coverslips and need to coat with poly-D-...

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Tips on freezing and thawing of cells

Sep 10 2004 12:32 PM | bioforum in Cell Culture

I am facing problems in thawing of cells. The procedure which we follow is- we take stock from liq. N2 then though it in water bath at 37 degree. Then we take medium in falcon (5ml) then add thawed cells dropwise into it. We then centrifuge it at 1500...

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