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How to avoid foaming during...
By michaelddorsey, Feb 18 2019 05:49 AM -
How to find promoter sequen...
By Kikogarcia, Nov 15 2018 01:29 AM -
Issues Might Meet in the Pr...
By Ashlay Donlad, Jun 30 2016 05:39 AM -
Can 18s gene be used as ref...
By Ashlay Donlad, Mar 28 2016 06:47 AM -
How to find promoter sequen...
By Ashlay Donlad, Feb 05 2016 05:57 AM -
Sonication Vs MNase for ChI...
By Ashlay Donlad, Feb 05 2016 05:13 AM
Latest Articles
Pinned How to avoid contamination in PCR?
PCR contaminations are like ghosts... you know they are there but you dont know WHERE?? I have followed the following with good results... Prepare your master mix in a separate room from your current location, somewhere PCR is not the main practice....
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How to convert g into rpm?
May 28 2009 07:05 PM | bioforum in Centrifugation
The relationship between revolutions per minute (RPM) and relative centrifugal force (xg) is: g = (1.118 × 10-5) R S2 where g is the relative centrifugal force, R is the radius of the rotor in centimeters, and S is the speed of the centrifuge in revolu...
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Handling RNA - How to avoid RNase contamination?
Dec 08 2009 09:31 PM | bioforum in RNA Methods
Hi Susan, I would second what tea-test has pointed out. Any tissue you process is going to be loaded with RNases (cells contain RNases). Any residual RNase present on your homogenizer is going to be very minor compared to what is already present in...
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How to coat coverslips before seeding cells?
Aug 18 2011 05:55 AM | jennycharlies in Cell Culture
jennycharlies Posted 18 August 2011 - 06:55 AM Hi, I am going to seed my cells onto coverslips inside the wells of a 12 well plate and was wondering what is the best way to coat the coverslips? I have 18mm coverslips and need to coat with poly-D-...
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How to avoid foaming during sonication in ChIP...
Feb 01 2009 01:33 PM | bioforum in ChIP Assay
ChIP - Foaming in sonication and other woes - <small> (Jan/27/2005 )</small>Hi all! I was wondering if anyone out there has got any trick to avoid foaming during sonication in the SDS lysis buffer for ChIP assays. Supossedly, placi...
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How to find promoter sequence for methylation s...
Aug 05 2009 07:43 PM | pcrman in Bioinformatics and Biostatistics
Many people have problem identifying or predicting the promoter sequence of a gene, or don't know how to get the actual sequence for analysis such as primer design, transcription factor binding site search, etc. Here I provide ways how I do these t...
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promoter, tss, sequence, database and 1 more...
Issues Might Meet in the Process of Using ELISA...
There are some issues that researchers might meet in the process of using ELISA Kits:
1. Positive results in negative control
2. High background across entire plate
3. Low absorbance values
4. High absorbance values for samples and/or positive control - absorbance does not go down as the sample is diluted down the plate
5. Inconsistent absorbance across the plate
6. Color develops slowly
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Elisa Kits, Creative Biomart
Can 18s gene be used as reference gene for RT-PCR?
Hi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can't...
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real time PCR, house keeping gene and 2 more...
Sonication Vs MNase for ChIP assay
Apr 14 2011 09:59 AM | Dukey in ChIP Assay
I am sure that this debate has been covered before, but I just wanted to share my own personal experiences with it. I am working with a TF and fairly recently switched to MNase. I initially tried MNase digestion out of pure curiousity, but I have to sa...
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How to get rid of PCR primer dimer
1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...
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