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Differences between MSP and...
By Doug55, Today, 03:40 AM -
How to avoid foaming during...
By Shad Doyle, Jun 30 2022 02:47 AM -
Issues Might Meet in the Pr...
By scottpeterss, Jun 22 2022 12:22 PM -
Differences between MSP and...
By scottpeterss, Jun 20 2022 08:34 AM -
How to convert g into rpm?
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Common causes for low RNA A...
By scottpeterss, Jun 17 2022 12:44 PM
Latest Articles
Pinned How to avoid contamination in PCR?
PCR contaminations are like ghosts... you know they are there but you dont know WHERE?? I have followed the following with good results... Prepare your master mix in a separate room from your current location, somewhere PCR is not the main practice....
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Differences between MSP and BSP methods
Mar 02 2005 12:16 AM | bioforum in Bisulfite Modification based Methods
Hello everyone I'm new here and I was wondering if I could get some assistance. In my research of the literature I thought the differences between the two methods were based on the proximity of the primers to the CpG sites and that BSP primers...
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How to avoid foaming during sonication in ChIP...
Feb 01 2009 01:33 PM | bioforum in ChIP Assay
ChIP - Foaming in sonication and other woes - <small> (Jan/27/2005 )</small>Hi all! I was wondering if anyone out there has got any trick to avoid foaming during sonication in the SDS lysis buffer for ChIP assays. Supossedly, placi...
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Issues Might Meet in the Process of Using ELISA...
There are some issues that researchers might meet in the process of using ELISA Kits:
1. Positive results in negative control
2. High background across entire plate
3. Low absorbance values
4. High absorbance values for samples and/or positive control - absorbance does not go down as the sample is diluted down the plate
5. Inconsistent absorbance across the plate
6. Color develops slowly
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Elisa Kits, Creative Biomart
How to convert g into rpm?
May 28 2009 07:05 PM | bioforum in Centrifugation
The relationship between revolutions per minute (RPM) and relative centrifugal force (xg) is: g = (1.118 × 10-5) R S2 where g is the relative centrifugal force, R is the radius of the rotor in centimeters, and S is the speed of the centrifuge in revolu...
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Common causes for low RNA A260/230 ratios
Jul 30 2013 01:38 PM | robradford in RNA Methods
Discussion on things that cause a low A260/230 ratio. Please add your comments.
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RNeasy, contamination, RNA and 1 more...
DNA migrate differently in agarose vs PAGE gel
Aug 15 2011 08:59 PM | pcrman in Electrophoresis
i am currently working a gene in leukemia. The mutant allele has been sequenced to contain 527 base pair. when i run an agarose gel to confirm, it did appear in the position approximately equal to 527 base pair according to the DNA ladder. HOWEVER, whe...
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How to find promoter sequence for methylation s...
Aug 05 2009 07:43 PM | pcrman in Bioinformatics and Biostatistics
Many people have problem identifying or predicting the promoter sequence of a gene, or don't know how to get the actual sequence for analysis such as primer design, transcription factor binding site search, etc. Here I provide ways how I do these t...
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promoter, tss, sequence, database and 1 more...
How to get rid of PCR primer dimer
1) Try the DMSO up to 5%. Some polymerases are ok with DMSO and others aren't. You never know until you try. 2) Try a two step PCR. There is a paper in Biotechniques about a two step PCR reaction but I don't have the reference with me sin...
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Can 18s gene be used as reference gene for RT-PCR?
Hi, I have done few real time PCR works recently, and I used the 18s gene for endogeneous control. Its Ct value was approximately 11 for every cDNAs used as template. Here, I have a question. Since 18s gene doesn't have poly A tail, it can't...
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real time PCR, house keeping gene and 2 more...
