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Pinned  How to avoid contamination in PCR?

Oct 02 2004 12:24 PM | bioforum in PCR

PCR contaminations are like ghosts... you know they are there but you dont know WHERE?? I have followed the following with good results... Prepare your master mix in a separate room from your current location, somewhere PCR is not the main practice....

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Differences between MSP and BSP methods

Hello everyone I'm new here and I was wondering if I could get some assistance. In my research of the literature I thought the differences between the two methods were based on the proximity of the primers to the CpG sites and that BSP primers...

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Sonication Vs MNase for ChIP assay

Apr 14 2011 09:59 AM | Dukey in ChIP Assay

I am sure that this debate has been covered before, but I just wanted to share my own personal experiences with it. I am working with a TF and fairly recently switched to MNase. I initially tried MNase digestion out of pure curiousity, but I have to sa...

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How to avoid foaming during sonication in ChIP...

Feb 01 2009 01:33 PM | bioforum in ChIP Assay

ChIP - Foaming in sonication and other woes - <small> (Jan/27/2005 )</small>Hi all! I was wondering if anyone out there has got any trick to avoid foaming during sonication in the SDS lysis buffer for ChIP assays. Supossedly, placi...

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How to coat coverslips before seeding cells?

Aug 18 2011 05:55 AM | jennycharlies in Cell Culture

jennycharlies Posted 18 August 2011 - 06:55 AM Hi, I am going to seed my cells onto coverslips inside the wells of a 12 well plate and was wondering what is the best way to coat the coverslips? I have 18mm coverslips and need to coat with poly-D-...

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How to convert g into rpm?

May 28 2009 07:05 PM | bioforum in Centrifugation

The relationship between revolutions per minute (RPM) and relative centrifugal force (xg) is: g = (1.118 10-5) R S2 where g is the relative centrifugal force, R is the radius of the rotor in centimeters, and S is the speed of the centrifuge in revolu...

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DNA migrate differently in agarose vs PAGE gel

Aug 15 2011 08:59 PM | pcrman in Electrophoresis

i am currently working a gene in leukemia. The mutant allele has been sequenced to contain 527 base pair. when i run an agarose gel to confirm, it did appear in the position approximately equal to 527 base pair according to the DNA ladder. HOWEVER, whe...

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Useful Dilution Techniques & Calculations

Mar 21 2009 02:33 PM | bioforum in General Lab Techniques

Today was a very bad day at the university... beside that some students answered in the quiz question that if you get blue color in Gram staining that means your bacteria is Gram negative :huh: I noticed most of the students including myself had troub...

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Handling RNA - How to avoid RNase contamination?

Dec 08 2009 09:31 PM | bioforum in RNA Methods

Hi Susan, I would second what tea-test has pointed out. Any tissue you process is going to be loaded with RNases (cells contain RNases). Any residual RNase present on your homogenizer is going to be very minor compared to what is already present in...

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Issues Might Meet in the Process of Using ELISA...

Jul 03 2013 04:00 PM | dynah in ELISA

There are some issues that researchers might meet in the process of using ELISA Kits:

1. Positive results in negative control

2. High background across entire plate

3. Low absorbance values

4. High absorbance values for samples and/or positive control - absorbance does not go down as the sample is diluted down the plate

5. Inconsistent absorbance across the plate

6. Color develops slowly

Read story →    4 comments    -----    Elisa Kits, Creative Biomart

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