Anybody have problems with stem cells culture and EB contamination? Lately I have been experiencing lots of contamination, my entire batches of HESC and EB have all died!! My hard work have all gone down the drain!!! :~( What could it be due to!!!???

Another things, is the b-fgf very crucial for the stem cell differentiation respressing? I seem to have like add abit less than expected


PLS HELP!!!!