BioForum > how to put in evidence protein after IP

Full Version: how to put in evidence protein after IP

BioForum > Methods Discussion > Protein and Proteomics > Protein Expression and Purification

gigi

Jul 9 2009, 05:53 PM

Hello there !!

So after many times, my IP is working is coming down clean

,

now I have a question for who has more experience than me.

how can I "stain' all the protein come down with the IP?

I tried with the ponceau but I don't think it's strong enough !!

is there any ways to put evidence the protein pulled down using an HRP method?

thank you for your help..

G.

gigi

Jul 10 2009, 01:45 PM

Nobody has an Idea ?!

cellcounter

Jul 10 2009, 02:04 PM

QUOTE (gigi @ Jul 10 2009, 02:45 PM)

Nobody has an Idea ?!

Silver staining and Sypro Ruby staining methods are more sensitive for protein detection.

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