Does anyone who has experience in handling of primary neuron of fly could provide me detail protocol? I also wonder what reagent you're using to digest the brains? What I'm using now is collagenase but it seems not very powerful...

thanks in advance...

Try looking here

http://www.pubmedcentral.nih.gov/articlere...i?artid=2556163

...or here...

http://www.jove.com/index/Details.stp?ID=200


Hope that helps!
-Carlton

Thanks a lot...