Anyone has optmised protocols for the meausurement of IL6, IL10 and TNF in human plasma.
At the moment I am using BD OptEIA abs but using an amplification system for the substrate to try to increase sensitivity. However still difficult to measuring in the lower range of detection, so any comments or experience welcome!!
Full Version: Human IL6, IL10, TNF
QUOTE (jviana @ Apr 25 2009, 09:19 PM) 
Anyone has optmised protocols for the meausurement of IL6, IL10 and TNF in human plasma.
At the moment I am using BD OptEIA abs but using an amplification system for the substrate to try to increase sensitivity. However still difficult to measuring in the lower range of detection, so any comments or experience welcome!!
At the moment I am using BD OptEIA abs but using an amplification system for the substrate to try to increase sensitivity. However still difficult to measuring in the lower range of detection, so any comments or experience welcome!!
Dear Sir/Madam, I don't know that you have completed the meausurement of IL6, IL10 and TNF in human plasma or not. If not, maybe some relative ELISA kit in our company can help you. Please leave more information for me to provide you the regarding information .Or you can write to me. my e-mail: cusabio@hotmail.com
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