Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?
Full Version: Amyloid beta labelling
QUOTE (BioSaint @ Jan 27 2009, 05:51 PM) 
Hell all,
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?
I have a question, do you use some special labels for amyloid beta peptide? I have to make sure that the conformation of the specific oligomer should not be affected. I got to see them in live cells.. Any suggestions?
That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?
That might be difficult. What exactly do you want to do? Study the movement of A-beta or simple localization?
[color="#000000"][/color] *** Starting with localization.. FITC is ok.. But any other stuff?
Have you thought about using GFP fused to A-beta? They may not work but they could also work.
Yeah, thought about it mate, but it may harm the aggregation pattern..
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