Full Version: Non Reducing SDS protein ladder??
Hi
I would like to know if “non reducing SDS protein ladder” is available???
Hey rick there are two options
1) make it yourself - which might not be feasable for all applications specialy when repeatability is needed and you make different stocks
2) a better option is that Bio-Rad provides concentrated marker solutions (low and high range) which has to be diluted with reduced sample buffer before loading. So instead of reduced you can add the non-reducing buffer and use this as the nr ladder.
Hope this helps
Best luck!!!
1) make it yourself - which might not be feasable for all applications specialy when repeatability is needed and you make different stocks
2) a better option is that Bio-Rad provides concentrated marker solutions (low and high range) which has to be diluted with reduced sample buffer before loading. So instead of reduced you can add the non-reducing buffer and use this as the nr ladder.
Hope this helps
Best luck!!!
hi
thanks
well i liked the secound suggestion will look into it....
thanks
well i liked the secound suggestion will look into it....
i can give you the calalogue numbers if u need!!!
QUOTE (Pradeep Iyer @ Nov 20 2009, 01:56 AM) 
2) a better option is that Bio-Rad provides concentrated marker solutions (low and high range) which has to be diluted with reduced sample buffer before loading. So instead of reduced you can add the non-reducing buffer and use this as the nr ladder.
you won't be able to trust the results if you don't run them as prescribed.
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