Hi.

I need a mitochondrial probe that is not mitochondrial membrane potential-dependent. Can someone help me? I want to estimate if there is a bigger number of mitochondria. However I don't want to use probes that are potential-dependent.

Thanks in advance.

Try here

There are some MitoTracker "not dependen" of DeltaPsi.
You can also do a inmunofluorescence against cytochrome c, COX1...Also calcein, but is dependent on PTP, orange NAO or you can express a mitoGFP.