Does anyone have any experience in choosing qRT-PCR housekeeping genes for neuronal cultures? We already have tried b-actin and GAPDH but are not happy with them so thinking of trying some others... any info would be of great help!
gfischer
Nov 9 2009, 12:07 PM
QUOTE (scistudent @ Nov 9 2009, 01:24 PM)
Does anyone have any experience in choosing qRT-PCR housekeeping genes for neuronal cultures? We already have tried b-actin and GAPDH but are not happy with them so thinking of trying some others... any info would be of great help!
An isoform of beta-tubulin may work well, 1 or 3 especially.
This is a "lo-fi" version of our main content. To view the full version with more information, formatting and images, please click here.