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Full Version: HKG for neuronal cultures...?
BioForum > Methods Discussion > Neuroscience
scistudent
Does anyone have any experience in choosing qRT-PCR housekeeping genes for neuronal cultures? We already have tried b-actin and GAPDH but are not happy with them so thinking of trying some others... any info would be of great help!
gfischer
QUOTE (scistudent @ Nov 9 2009, 01:24 PM) *
Does anyone have any experience in choosing qRT-PCR housekeeping genes for neuronal cultures? We already have tried b-actin and GAPDH but are not happy with them so thinking of trying some others... any info would be of great help!


An isoform of beta-tubulin may work well, 1 or 3 especially.
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