Is it true that by adding a few nucleotides to the restriction sites of some endonucleases you will increase its efficiency?
I think I heard that in a lecture, but my Pi have never heard of it...so, I am confused now....
Full Version: Restriction enzymes
HI
Is it true that by adding a few nucleotides to the restriction sites of some endonucleases you will increase its efficiency?
I think I heard that in a lecture, but my Pi have never heard of it...so, I am confused now....
Is it true that by adding a few nucleotides to the restriction sites of some endonucleases you will increase its efficiency?
I think I heard that in a lecture, but my Pi have never heard of it...so, I am confused now....
You add the extra bases to the end ofteh RE site ,to give hte enzyme something to hold onto. Check out the NEb website and see how many extra bases are required to improve your Re's efficiency.
Thanks!
QUOTE (swanny @ Nov 3 2009, 06:08 AM) 
You add the extra bases to the end ofteh RE site ,to give hte enzyme something to hold onto. Check out the NEb website and see how many extra bases are required to improve your Re's efficiency.
Tat was informative.. although i don work with RE's, this came as an interesting fact.. thanx for the thread canar
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