Hi everyone,
I'm researching a secreted protein in culture medium from cell lines of prostate cancer. I run a WB without problems, however I need a secreted protein to normalize the results and loading control (like actin in protein extract).
Are there any protein that always be released to culture medium?
Thank you!
Valentina
Full Version: Secreted protein for loading control
I have read about others using erk (extracellular signal-regulated kinase) as such a loading control.
See Coban 2005, J. Leukocyte Biology 78:647-655. Also see Dittmer 2006, Electrophoresis 27, 2844-2845.
Cheers,
Matt
See Coban 2005, J. Leukocyte Biology 78:647-655. Also see Dittmer 2006, Electrophoresis 27, 2844-2845.
Cheers,
Matt
QUOTE (dzasp @ Oct 29 2009, 06:03 AM) 
Hi everyone,
I'm researching a secreted protein in culture medium from cell lines of prostate cancer. I run a WB without problems, however I need a secreted protein to normalize the results and loading control (like actin in protein extract).
Are there any protein that always be released to culture medium?
Thank you!
Valentina
I'm researching a secreted protein in culture medium from cell lines of prostate cancer. I run a WB without problems, however I need a secreted protein to normalize the results and loading control (like actin in protein extract).
Are there any protein that always be released to culture medium?
Thank you!
Valentina
Loading controls are fake-prone.
I would recommend you simply performing ponceau stain, scan and lane densitometry.
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