Hi all,
I have to titrate some fluorochrome-conjugated antibodies for flow cytometry experiments and I'd like to solve some doubts.
If I titrate an antibody with a cell concentration of 2*10^5 cell/100ul, do I need to titrate it again when using a cell
concentration of 5*10^5-10^6 cell/100ul?
Thanks a lot!
Full Version: Antibody titration and Cell number
QUOTE (borriello.87 @ Oct 18 2009, 05:00 AM) 
Hi all,
I have to titrate some fluorochrome-conjugated antibodies for flow cytometry experiments and I'd like to solve some doubts.
If I titrate an antibody with a cell concentration of 2*10^5 cell/100ul, do I need to titrate it again when using a cell
concentration of 5*10^5-10^6 cell/100ul?
Thanks a lot!
I have to titrate some fluorochrome-conjugated antibodies for flow cytometry experiments and I'd like to solve some doubts.
If I titrate an antibody with a cell concentration of 2*10^5 cell/100ul, do I need to titrate it again when using a cell
concentration of 5*10^5-10^6 cell/100ul?
Thanks a lot!
I don't think so just double your antibody amount but then double as well your volume then you'll be almost in the same concentrations
This is a "lo-fi" version of our main content. To view the full version with more information, formatting and images, please click here.