glbinbin
Oct 15 2009, 04:29 PM
I used MTT assay to get known of cell proliferation. I dont know which OO values to be used. Single-wavelength 490nm? 570nm? 630nm? Or dual-wavelength?
Please give me some advice, thanks.
jah
Oct 16 2009, 04:48 AM
Formazan, the product of MTT reduction, has an absorbance at 493nm in when solubilized in DMSO, so 490nm would be your best choice from the filters you mentioned.