BioForum > Doing mutagenesi for large plasmid or articificial chromosome

Full Version: Doing mutagenesi for large plasmid or articificial chromosome

BioForum > Methods Discussion > Molecular Cloning

krystle

Oct 11 2009, 11:14 PM

Hi, does anyone know the kind of strategy to perform mutagenesis on large plasmid of around 30kb (20+kb insert) or on articifial chromosomes?
I know for large plasmids there is a XL mutagenesis kit for plasmid >8kb from strategene, but i'm not sure if it will work plasmids up to 30kb. If anyone can direct to any resources it will be great. Thanks in advance.

Regards,

Warren

Oct 12 2009, 07:15 PM

I would subclone a smaller fragment into a regular vector, do the mutagenesis, then subclone it back in.

Warren..

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