hi,
I would like to know how important the time constant in electroporation of ES cells.

I'm using biorad gene pulser -II for electroporation. I use 240 volts and 500micro farady for electroporation. Every I get a time constant 8.2-8.8. Is this normal?

thanks

best
mm

I could be wrong, but I think the time constant can vary with the buffer you are electroporating cells in.