reconstitution of lyophilized enzyme (soybean 15-lipoxygenase-1, Sigma L7395), how to? ive tried using sodium borate buffer (.2M pH 9) but it doesnt fully dissolve in it. any suggestions?
swanny
Oct 6 2009, 09:55 PM
Does Sigma have any suggestions? Try the technical specialists in your area.
mdfenko
Oct 7 2009, 10:27 AM
the information sheet says that it is soluble to 0.1mg/ml. are you trying to make it too concentrated?
it may take a little time to fully solubilize.
DaveD
Nov 18 2009, 10:23 PM
Solubility is usually a matter of pH, salt and something else (glycerol, BME, EDTA etc.). Got to play around with these factors and identify the sweet spot (or look it up in a publication that goes into a description of the buffer). My 2 ct Dave Protein Solubility kits
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