Full Version: Dpn1 enzyme
Can anybody tell me what's the function of this enzyme? Thanks
It's often used after PCR to get rid of template DNA. DpnI only cuts methylated DNA, so PCR products are untouched. Template DNA on the other hand gets cut to shreds. This is very important if your template DNA is a plasmid and you want to do some cloning and transformation....
QUOTE (swanny @ Sep 30 2009, 04:08 PM) 
It's often used after PCR to get rid of template DNA. DpnI only cuts methylated DNA, so PCR products are untouched. Template DNA on the other hand gets cut to shreds. This is very important if your template DNA is a plasmid and you want to do some cloning and transformation....
Oh I see. Thank you very much for your reply!
This is a "lo-fi" version of our main content. To view the full version with more information, formatting and images, please click here.