I need to find a way to optimize the IHC in MCAO brain.

I got some problems using the criostat (10 micron) in MCAO brains, my impression is that the tissue after stroke induction become fragile and this make me difficult to get good slices .
I looking for an alternative protocol able to get me thick slices to treat with anti-bodyes.

thanks

tizzy

In what way are the tissue sections poor? Are they wrinkling, cracking, something else? If you can describe the protocol in more details, that would help us provide a solution.

Cheers,
-Carlton