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  1. Pinned: Problem in culturing MCF-7 cell line! (16 replies)
  2. Pinned: Electrophoresis (8 replies)
  3. Pinned: Apoptosis Protocol (3 replies)
  4. Pinned: Population doubling time of keratinocyte,etc. (13 replies)
  5. Pinned: help with cryopreservation (10 replies)
  6. Pinned: MTT assay problems, while testing anticancer activity (16 replies)
  7. Pinned: virus vector for transfection (7 replies)
  8. Pinned: help - stable transfection clone pick up (14 replies)
  9. Pinned: Freezing and thawing of cells (24 replies)
  10. J774.2 Macrophages (0 replies)
  11. How well do macrophages respond to DMSO? (0 replies)
  12. Marker do discriminate cell cycle phases (0 replies)
  13. how to make nonadhensive cells attach to coverslips without affecting cellular s (1 reply)
  14. Freezing THP-1 (8 replies)
  15. stable cell line generation (3 replies)
  16. Bone marrow storage (1 reply)
  17. Sterilization (5 replies)
  18. Liquid nitrogen problem (2 replies)
  19. Acridine orange (3 replies)
  20. protein isolation (3 replies)
  21. Can you remove OCT from tissue? (1 reply)
  22. immunofluorescence and western blotting (3 replies)
  23. Human adult cardiac fibroblasts (0 replies)
  24. Cell Synchronization (1 reply)
  25. Mitochondrial probes (1 reply)
  26. Floating cells...pls help (6 replies)
  27. Problems with HEK293 cells in 96 well plate format (1 reply)
  28. Using plastic for microscopy? (3 replies)
  29. Immunocytochemistry for death cells (0 replies)
  30. Scratch/Wound Healing Assay Substrate? (9 replies)
  31. self activation of BD (0 replies)
  32. Stem cell review (0 replies)
  33. Murine bone marrow progenitors in cuture (3 replies)
  34. 12 uM transwell (2 replies)
  35. infection efficiency (4 replies)
  36. fibroblast primary culture (8 replies)
  37. MTT assay is not working (9 replies)
  38. mouse liver cell line? (1 reply)
  39. cross talk/bleed in confocal (3 replies)
  40. Small flourescent tag (1 reply)
  41. Expression of a GFP-tagged protein (2 replies)
  42. MDCK cells vacuolation (4 replies)
  43. Cells suddenly died after changing the medium... why? (4 replies)
  44. How long can viably frozen cells stay good in -80 degree? (5 replies)
  45. mutations & aging etc (1 reply)
  46. ROS measurement (8 replies)
  47. Mitochondrial membrane potential gain (1 reply)
  48. Total ROS measurement protocol (1 reply)
  49. Freezing U937 (1 reply)
  50. CM definition (0 replies)
  51. lentiviral system: cloning strategy (2 replies)
  52. What does fibronecting coating do? (2 replies)
  53. Transfection of NIH/3T3 (2 replies)
  54. Fas death receptor inhibitor (0 replies)
  55. calcium measurment by Fluo-3 AM (0 replies)
  56. Moved: plasmid DNA lab question (-- replies)
  57. LysoSensor Blue DND-167 (1 reply)
  58. DAG levels in hepatocytes (0 replies)
  59. Cell Yields from Adult Rat Hippocampal Cultures (0 replies)
  60. Renca cells (murine RCC) (0 replies)
  61. Possible contamination - suggestions? (7 replies)
  62. Need help to select products of migration and invasion assay from BD Biosciences (0 replies)
  63. How to evaluate SV40 expression level (4 replies)
  64. About T-antigen and tumor cell lines (2 replies)
  65. changing cell shape (1 reply)
  66. Axiovision software (0 replies)
  67. Cell Fixation (3 replies)
  68. lymphoblast studies (4 replies)
  69. expression of SV40 in luciferase assay (3 replies)
  70. XTT (0 replies)
  71. V5 tag-fused protein in adenoviral vector for immunofluorescence (0 replies)
  72. Myeloblast isolation (0 replies)
  73. Stable Cotransfection in CHOK1 (0 replies)
  74. Determination of Mitochondrial Mass (0 replies)
  75. Mitosis at an embryogenesis. Whether there is a crossing-over? (0 replies)
  76. Luciferase assay (2 replies)
  77. How to culture adherent cell lines on tosyl activated magnetic beads (0 replies)
  78. my cell line doesnt change the colour of the media (5 replies)
  79. XTT assay (0 replies)
  80. Working gene-coding plasmid does not produce transcript or protein (2 replies)
  81. X-Y plane confocal imaging (1 reply)
  82. Need advice on Adhesion Assay (5 replies)
  83. Differentiating Thp-1 into macrophage (13 replies)
  84. about measuring luciferase activity in living cells (8 replies)
  85. what chemical can easily induce cell senescene? (0 replies)
  86. internal control (4 replies)
  87. RAW264.7 cells+nitric oxide determination (6 replies)
  88. Cell isolation (1 reply)
  89. MDA-MB-175 (4 replies)
  90. what is the point of serial dilution??? (2 replies)
  91. Transfection (7 replies)
  92. Minimum transfection incubation time (2 replies)
  93. plasmid size limit for transfection? (0 replies)
  94. 3D Collagen Matrix Polymerization (1 reply)
  95. trouble to grow cells on top of coverslip (6 replies)
  96. How to increase IF signal in acetone/methanol fixed cells? (5 replies)
  97. exosome purification (1 reply)
  98. Problems with stable transfection (3 replies)
  99. Tumour Spheroid models (0 replies)
  100. Hanging Drop Aggregation Assay (0 replies)
  101. SY5Y protein content (0 replies)
  102. is a spacer needed for His tag antibody detection? (0 replies)
  103. preadipocyte 3T3-L1 cells question? (1 reply)
  104. Osteoclasts (4 replies)
  105. Outer membrane preparation protocol. (1 reply)
  106. Methanol toxicity in cell culture (15 replies)
  107. Immunocytochemistry (3 replies)
  108. timing of apoptosis events (0 replies)
  109. effect of calcium and magnesium during PBMC isolation? (1 reply)
  110. Cell counting issues (5 replies)
  111. Has anyone done a comparison between LDH and Adenylate kinase cytotoxicity assay (2 replies)
  112. MC3T3-E1 osteogenic differentiation (1 reply)
  113. INS-1 cell line (1 reply)
  114. Can anyone suggest a good LDH assay method? (0 replies)
  115. Caspase 3 Assays (2 replies)
  116. research about senescence (2 replies)
  117. How can I avoid the bubbles in the glass slide? (1 reply)
  118. Annexin V staining in combination with other antibodies (4 replies)
  119. Cell Brakeage...how will I know (1 reply)
  120. LNCaP xenografts (1 reply)
  121. monocyte devired macrophage (1 reply)
  122. Contamination in Transfection Agent (7 replies)
  123. Improving transient CHO protein expression (0 replies)
  124. HepG2 siRNA transfection (1 reply)
  125. problem with Mono Mac1 differentiation (3 replies)
  126. 1,25 Vit D3 reactivity (0 replies)
  127. Transient expression vector (11 replies)
  128. N-Acetylcysteine (2 replies)
  129. Anybody ever use whole blood plasma serum stimulate cells? (2 replies)
  130. Does anybody have CD40L+ L cells (human CD40 Ligand transfected mouse fibroblast (0 replies)
  131. CHO or VERO? (2 replies)
  132. Freeze down primary monocytes? (1 reply)
  133. Best lentiviral vector (2 replies)
  134. 90% confluency (12 replies)
  135. Oxygen diffusion through cell culture medium (2 replies)
  136. Vibrating microtome vs Tissue slicers (1 reply)
  137. fibroblasts transfection (1 reply)
  138. Paraformaldehyde fixation! (1 reply)
  139. How much sonication power needed? (2 replies)
  140. Cytospin (1 reply)
  141. CM-H2DCFDA for Microscopy (3 replies)
  142. Role of Butyrate in Transfection (0 replies)
  143. Lipofectamine transfections once working now dying (5 replies)
  144. Neutrophil separation (0 replies)
  145. Variable expression of antigen in various subcellular locations - Any ideas why? (2 replies)
  146. Lentivirus expression system (10 replies)
  147. Problem with stable overexpression cell lines (3 replies)
  148. Secretory protein? (2 replies)
  149. protein complex and siRNA (2 replies)
  150. opti-mem as a serum starvation media (1 reply)
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