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BioForum > Methods Discussion > Protein and Proteomics
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  1. Pinned: Western Blotting background troubleshooting (14 replies)
  2. Pinned: semi-dry western conditions (13 replies)
  3. Pinned: problem with amonium sulfate precipitation (13 replies)
  4. Pinned: x-ray films vs direct imaging (10 replies)
  5. Pinned: protein expression in Pichia pastoris (22 replies)
  6. Pinned: SDS-PAGE (13 replies)
  7. Pinned: SDS-PAGE running strangely (19 replies)
  8. Pinned: immunoprecipitation protocol (2 replies)
  9. Pinned: Current Protocol by John Wiley & Son (0 replies)
  10. Pinned: His tag protein purification problem (15 replies)
  11. Out of expected protein size in SDS-PAGE (8 replies)
  12. Sugar solution had changed colour (8 replies)
  13. Incomplete denaturing with 8M urea? (1 reply)
  14. Q about the housekeeping gene (0 replies)
  15. Co-IP using magnetic beads, non-specific binding (4 replies)
  16. Microplate Bradford assay (4 replies)
  17. Protein quantification from paraformaldehyde-fixed cells (2 replies)
  18. Protein Cross-linking (1 reply)
  19. HA-TAG (2 replies)
  20. Recycling coomassie blue (5 replies)
  21. storing WB blots in PBS-tween (7 replies)
  22. precipitation of proteins from stained cells (6 replies)
  23. Bradford quantitatn problem - precipitation (1 reply)
  24. cAMP assay kit (0 replies)
  25. BSA content in Fetal Bovine Serum? (0 replies)
  26. problem in the GST fusion protein purification (4 replies)
  27. Storage of overexpressed protein (4 replies)
  28. HELP_Bradford and protein problems (1 reply)
  29. simple pipetting problem (1 reply)
  30. Bromophenol blue vs. "Coomassie G250" (2 replies)
  31. Co-immunoprecipitation (10 replies)
  32. Weird BSA standard curve for Bradford assay (5 replies)
  33. PDI western blot (5 replies)
  34. Concentrating Protein (7 replies)
  35. how to deal with protein precipitates during dialysis (1 reply)
  36. collagen type I ELISA (1 reply)
  37. mTOR (4 replies)
  38. Staining of GLycoproteins in IEF (4 replies)
  39. ideas for Fluorescent tagging of 7Kda bacterial protein. (1 reply)
  40. Extract collagen from rat tail (in lab) (1 reply)
  41. collagen extraction (1 reply)
  42. Storing Purified Proteins (7 replies)
  43. Qubit Protein Quantitation Issues (1 reply)
  44. iTRAQ cost compared to a DIGE approach (0 replies)
  45. Protein extraction from plant leaves, bioactivity (1 reply)
  46. BN-PAGE (2 replies)
  47. Extracellular protein isolation (0 replies)
  48. LC-MS protocol (7 replies)
  49. SDS PAGE: sample buffer 2-ME or DTT?? (1 reply)
  50. SDS PAGE:no of lanes senstivity?? (1 reply)
  51. Protein Disappeareance (1 reply)
  52. Bradford assay+high urea (3 replies)
  53. Western blotting and immunofluorescence results don't correspond to each oth (2 replies)
  54. Collagen Quantification (0 replies)
  55. Coomassie blue before protein transfer (9 replies)
  56. 2D gel (1 reply)
  57. protein size change after western blot (2 replies)
  58. Glycoproteins on SDS-PAGE (3 replies)
  59. Expression for plant proteins in E.coli (0 replies)
  60. Increase in protein size (5 replies)
  61. step-wise dialysis? (11 replies)
  62. RIPA buffer pH? (1 reply)
  63. Western blot protocol (2 replies)
  64. western blot problem: cell culture VS mouse tissue (4 replies)
  65. Immunoprecipitation sucess (0 replies)
  66. identify kinase/phosphatase (1 reply)
  67. His tag Protein Elution (2 replies)
  68. How to analyze the ingredients of protein bands in native-PAGE gel? (3 replies)
  69. net charge on protein (1 reply)
  70. SDS-page and enzyme activity (1 reply)
  71. co-IP trouble shooting (0 replies)
  72. Overexpression experiment controls (2 replies)
  73. Problem with eGFP fusion protein (0 replies)
  74. "wrong" molecular weights (1 reply)
  75. Membrane protein isolation (1 reply)
  76. Measuring protein aggregation using Non-denaturing PAGE (3 replies)
  77. Competition between two proteins using pulldown assay (0 replies)
  78. Pierce Direct IP kit (0 replies)
  79. how better is 7Cm 2D gel electrophoresis for separation (1 reply)
  80. starry sky western blot... lots of spots. (5 replies)
  81. help for using alkaline phosphatase fusion protein to detect the protein inter (0 replies)
  82. proteomics (1 reply)
  83. bacteria lysis (1 reply)
  84. Difference between Pro-Q-Emerald 300 and Pro-Q-Emerald 488 (1 reply)
  85. Protein Sample Loading Buffer (3 replies)
  86. One treatment not running (0 replies)
  87. PBS or TBS (4 replies)
  88. pKa values of the protein (1 reply)
  89. V5 tag for pull down assay (0 replies)
  90. Plasmids for protein expression in mammalian cells (2 replies)
  91. affinity purification and proteomics (1 reply)
  92. problem with immunoprecipitation (0 replies)
  93. Lyophilised protein (3 replies)
  94. Isolation of plasma membrane, focal adhesion and cytoskeleton proteins (0 replies)
  95. cell lysis after induction (1 reply)
  96. Get rid of particles on gel surface after SYPRO-Ruby staining (1 reply)
  97. What proteins stick to ProteinG on preclearing? (3 replies)
  98. Post translational modification (1 reply)
  99. A databse linking SNPs to functional consequences? (2 replies)
  100. Problem in protein expression (2 replies)
  101. Protein qauntitation with nanodrop (1 reply)
  102. Immunoprecipitation - Bands in negative control (2 replies)
  103. purpose of input in IP (1 reply)
  104. 2d gel analysis software??? (6 replies)
  105. western blotting problem with detection (2 replies)
  106. help~ how concentrate protein solution in large-scale (2 replies)
  107. Protein purification (0 replies)
  108. extracting protein from trizol (2 replies)
  109. help!! immunoprecipitation (5 replies)
  110. Do I need to dilute my mouse tissue lysates for micro-BCA? (1 reply)
  111. protein precipitation (0 replies)
  112. competitive binding (1 reply)
  113. Western blot (0 replies)
  114. Difference between core and terminal glycosylation (0 replies)
  115. Digitonin Pellet (0 replies)
  116. Overblocking Western? (5 replies)
  117. FPLC is behaving strangely (11 replies)
  118. Overxepression of Tagged construct for co IP (0 replies)
  119. How strong is the "passive absorption"interaction? (0 replies)
  120. Phospho-Elk1 specific Antibody...which band?? (3 replies)
  121. Zymography and Sucrose (1 reply)
  122. Re-dissolving of precipitated protein?? (1 reply)
  123. Protein purification from Insect media (0 replies)
  124. sticky proteins in co-IP (1 reply)
  125. Purifying a strange native protein (6 replies)
  126. nuclear extract -- HEPES pH?! (1 reply)
  127. Protein G Sepharose beads preparation (2 replies)
  128. Determining a protein's antigenic property (4 replies)
  129. BCA assay for reducing ends (2 replies)
  130. Loading sample in FPLC loops (7 replies)
  131. Blurred low molecular weight bands in SDS-PAGE (1 reply)
  132. isolation of proteinase from helicoverpa gut (0 replies)
  133. Best yeast expression system (1 reply)
  134. FPLC and Protein Chromatography (8 replies)
  135. Why most commercial protease inhibitor cocktails do not contain EDTA? (5 replies)
  136. Purification of virus particles for analysis (0 replies)
  137. Improving transient CHO protein expression (2 replies)
  138. help! non-specific background in Co-IP (5 replies)
  139. insoluble protein fraction (1 reply)
  140. Nonspecific Binding on Western Blot (4 replies)
  141. stacking gel (4 replies)
  142. Hyaluronic Acid Binding Protein 1 (HABP1) (2 replies)
  143. Newbie question: Stability of protein lystates (2 replies)
  144. EMSA with pET-His protein (0 replies)
  145. Human collagen type I molecular weight (3 replies)
  146. nonspecific immunoprecipitation control (2 replies)
  147. isolation of membrane associated proteins (2 replies)
  148. Reduced glutathione removal (0 replies)
  149. Rabbit IgG purification (5 replies)
  150. protein extraction (5 replies)
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