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BioForum > Methods Discussion > Protein and Proteomics
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  1. WB for phosphorylated protein complex in rat intestinal tissue (2 replies)
  2. Slimy protein lysates (3 replies)
  3. Detergent concentration for nuclear membrane dissolving (2 replies)
  4. HPLC baseline problem (1 reply)
  5. Instant Blue / Stain (3 replies)
  6. Tissue sonication for RNA extraction (1 reply)
  7. brown pigment attached to the protein (3 replies)
  8. protein precipitation (1 reply)
  9. Subcellular fractionation (6 replies)
  10. Protein extraction from mouse organs (6 replies)
  11. CO-IP problem: non specific prot binding to G beads (11 replies)
  12. Immunoprecipitation vs conventional WB (7 replies)
  13. How to prepare plasma-membrane fraction? (6 replies)
  14. part of protein sequence missing (4 replies)
  15. How to define the domans in protein sequence (3 replies)
  16. MALDI data (3 replies)
  17. pGEX (2 replies)
  18. Problems on IEF for 2DE (2 replies)
  19. protein quantitation (3 replies)
  20. Mouse Urinary Protein Quantification (2 replies)
  21. Phosphoproteins (1 reply)
  22. strange double band in electrophoretic pattern (2 replies)
  23. How to detect protein-cytoskeleton interaction? (1 reply)
  24. difference in techniques (1 reply)
  25. Immunoprecipiation desperate request! (3 replies)
  26. Western blot stripping problem (6 replies)
  27. ACh/AChE assay and synaptic vesicles (0 replies)
  28. smear of loading buffer observed (1 reply)
  29. How to load 60 micro-litre sample to a single well in SDS-PAGE? (6 replies)
  30. there seems no induction of my recombinant protein (0 replies)
  31. All negative effects of His-Tag and c-myc epitope? (0 replies)
  32. Protein Homogenization (1 reply)
  33. extraction of extracellular protein (0 replies)
  34. detection of protein phosphorylation using M.S. (4 replies)
  35. trouble with PLC-gamma blot (0 replies)
  36. keratin extraction (0 replies)
  37. recombinant protein lost immunological activity (1 reply)
  38. MAPK P38 (6 replies)
  39. Western Blotting Spotting (5 replies)
  40. No peak for gel filtration for soluble protein (1 reply)
  41. Ni-NTA problem (7 replies)
  42. Protein with His6 tag (2 replies)
  43. Good buffer needed! (7 replies)
  44. His-tag protein purification (5 replies)
  45. Protein produced with brown color? (1 reply)
  46. His-Tag purification using Qiagen Ni-NTA spin column (2 replies)
  47. can I freeze the gel slice for protein sequencing (0 replies)
  48. Utility of the K2HPO4 (1 reply)
  49. Storing a protein membrane overnight? (3 replies)
  50. specificity of antibody in protein microarray (0 replies)
  51. question about the protein extraction (0 replies)
  52. How to identify a protein that binds a defined DNA sequence (4 replies)
  53. total stat5 levels in mammary gland (1 reply)
  54. Reuse antibodies for further incubation in Western blot (5 replies)
  55. immunoprecipitation (6 replies)
  56. sensitivity and staining specificity of sypro vs. proQ (0 replies)
  57. Western blot - Stripping and Reprobing (8 replies)
  58. Protein purification (3 replies)
  59. Vector with T5-promotor in Bl21/DE3-system (1 reply)
  60. p-Smad2 problems in western blot (1 reply)
  61. Plant protein sample cleanup for 2D gel (0 replies)
  62. ABC technique with Western blot (0 replies)
  63. Western blot problem (5 replies)
  64. Trouble with Western Blot Transfer (Poor transfer) (3 replies)
  65. Western Blot (3 replies)
  66. Phosphorylation not affecting SDS-PAGE mobility (6 replies)
  67. GST tag (0 replies)
  68. coomassie blue stained gel (3 replies)
  69. electroblotting (1 reply)
  70. phospho antibody detect untreat lane (6 replies)
  71. Extraction protein from soil (0 replies)
  72. How to improve yield of IP (2 replies)
  73. Nuclear protein extract (Scrape or Trypsinize)? (1 reply)
  74. Need protocol for SDS-Page on protein extracted from soil (0 replies)
  75. SDS-PAGE question (3 replies)
  76. I need some help for 2D electrophoresis (0 replies)
  77. Western Blot "Strange Results" Please help (4 replies)
  78. microtiter plate (2 replies)
  79. let me have a simple test for detecting phosphoproteins (5 replies)
  80. band spread in western (9 replies)
  81. Protocol for IEF (1 reply)
  82. SDS-PAGE of protein from membrane fraction (1 reply)
  83. the protein band disappear in the second dimension (2 replies)
  84. molecular weight markers (3 replies)
  85. Antibiotic use in Pichia cultures (1 reply)
  86. luciferase concentration? (5 replies)
  87. improving phospho-specific antibodies-how I did it (0 replies)
  88. MSI Ultrafuge 10K columns (0 replies)
  89. phosphopeptide! (0 replies)
  90. I need help for extraction of soil's proteins (0 replies)
  91. Immunoprecipitation (2 replies)
  92. Protein degradation (1 reply)
  93. HPLC problem (0 replies)
  94. apolipoprotein E identification via Westerns (0 replies)
  95. Glycosil transferase supplier (0 replies)
  96. HPLC (1 reply)
  97. isolation of nuclear protein (1 reply)
  98. protocol for Immunoprecipitation and 2DE (2 replies)
  99. IgG contamination of immunoprecipitated proteins (1 reply)
  100. Polymerization SDS Gel (7 replies)
  101. Protein degradation in media (beculovirus expression) (0 replies)
  102. Removing Exonuclease From Protein Prep (0 replies)
  103. What are Na3VO4 and beta-glycerophosphate for in lysis buffer (11 replies)
  104. gradient gel? (0 replies)
  105. Transferring a stained gel for western blot (0 replies)
  106. Cleaved GST fusion protein retained on beads! (4 replies)
  107. Far Western again... (1 reply)
  108. NOC - A program for protein structure explorer (1 reply)
  109. Protocol of protein denaturation by SDS (0 replies)
  110. Signal peptide - periplasmic protein (2 replies)
  111. Wet Transfer Problem (1 reply)
  112. WGA coupling with CNBr-activated sepharose 4B (1 reply)
  113. Western blot detection other than antibody? (5 replies)
  114. another 6his protein purification problem.... (0 replies)
  115. Nonspecific phosphatase (0 replies)
  116. Far western (3 replies)
  117. Sample Buffer Recipe (Laemmili) (4 replies)
  118. Plant cell wall protein (1 reply)
  119. loading control for western blots (1 reply)
  120. Bacterial Periplasmic protein localization (4 replies)
  121. protein precipitation after IMAC (1 reply)
  122. Preparation of competent cells of pichia (1 reply)
  123. measuring protein concentration (5 replies)
  124. Solubility ribosomal proteins (4 replies)
  125. membrane protein purification (1 reply)
  126. Purification through the Ni-NTa column (1 reply)
  127. expression of small peptide (2 replies)
  128. Protein extraction from rat liver tissue (1 reply)
  129. Total phosphorus determination (0 replies)
  130. ion-exchange chrom. under denature conditions (2 replies)
  131. protein expression (3 replies)
  132. elution sample form Mono Q column (4 replies)
  133. TCA precipitation (0 replies)
  134. problem in protein expression (0 replies)
  135. Cardiac Troponin I purification (0 replies)
  136. Error in SDS PAGE (1 reply)
  137. aggregation of purified proteins (3 replies)
  138. separated two protein (4 replies)
  139. Protien expression (3 replies)
  140. Help: About the synthesis of protein bead. (0 replies)
  141. quesiton about GST fusion protein (1 reply)
  142. A280 (1 reply)
  143. protein solubility (1 reply)
  144. protein dimerisation (1 reply)
  145. Problems with phospho-MAP and phospho-FAK (1 reply)
  146. Protein expression problems (4 replies)
  147. How long can 8M urea solution be stored? (0 replies)
  148. Non specific band at specific level & poor expression (0 replies)
  149. What is the T7 Tag, Hsv Tag, S Tag??? (0 replies)
  150. Lysing/Trypsinising for Phosphorylated protein measure? (0 replies)
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