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BioForum > Methods Discussion > Protein and Proteomics > Protein Expression and Purification
  1. Ni-NTA beads and dialysis tubing (3 replies)
  2. More than one protein induced (4 replies)
  3. Protein aggregates (0 replies)
  4. water withdrawal with PEG20.000 (1 reply)
  5. Troubleshooting GST purification (4 replies)
  6. protein expression (15 replies)
  7. Amount of inoculum? (3 replies)
  8. Role of imidazole in lysis buffer (4 replies)
  9. protein expression (temperature issue) (14 replies)
  10. BL21A or simliar (2 replies)
  11. Protein analog (1 reply)
  12. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? (2 replies)
  13. radiolabeling and co-IP with invitrogen dynabeads (0 replies)
  14. How do I get rid of this contaminant? (7 replies)
  15. how to add NLS to the protein (0 replies)
  16. Can GST-tag be separated from GST-fusion protein (0 replies)
  17. Help with T7RNAP purification (0 replies)
  18. Protein Extraction from blood/buffy coat (0 replies)
  19. membrane protein extraction buffer (1 reply)
  20. Truncated or isomer protein (0 replies)
  21. Problem with Protein elution using GST sepharose 4B (1 reply)
  22. Expression in yeast (0 replies)
  23. What about the Baculovirus expression system? (0 replies)
  24. how to isolate insoluble protein in bacterial induction? (4 replies)
  25. 293T electroporation (0 replies)
  26. pSecTag Expression of a protein with Signal Anchor (0 replies)
  27. protein is degraded after purification (2 replies)
  28. GST-fusion protein purification (9 replies)
  29. Desalting protein (7 replies)
  30. Problems purifying a protein expressed in Pichia (0 replies)
  31. Low expression: What else can I do? (9 replies)
  32. eluting antigen (0 replies)
  33. Immunoprecipitation (12 replies)
  34. Plasmid instability in E.coli BL21? (3 replies)
  35. Checking For Protein Expression (3 replies)
  36. Cleaving Fusion Proteins (0 replies)
  37. Couple questions about GST purification (16 replies)
  38. Plyse cell and DH5a (1 reply)
  39. Express bacterial gene into Pichia pastoris system. (0 replies)
  40. Quality of aliquot for SDS-page in protein purification (1 reply)
  41. Inducible expression in human cell line (3 replies)
  42. Electroelution with no electroeluter (3 replies)
  43. reusing Ni NTA (2 replies)
  44. primer design for pBAD topo expression kit (2 replies)
  45. Newbie question: how to determine protein concentration after IP? (8 replies)
  46. Antibody-beads crosslink (5 replies)
  47. dnase treatment before his-tag purification (3 replies)
  48. Storage of Nicotinic Acid in solution (0 replies)
  49. Changing dialysis buffer every half hour and then dialysis ON (0 replies)
  50. Expression of a GFP-tagged protein (0 replies)
  51. Low Thrombin Cleavage Efficiency Problem (1 reply)
  52. Is it possible? Harsh sonificiation conditions cause degradation of my protein (3 replies)
  53. protein aggregation problem (2 replies)
  54. Throbin cleavage declines with older protein? (1 reply)
  55. High pressure on Ni-NTA beads (2 replies)
  56. protein extraction using trizol (1 reply)
  57. affinity column problems (1 reply)
  58. Trypsin Expression and Purification (3 replies)
  59. How to elute this protein?! (5 replies)
  60. seeking help in stable cell line generation (3 replies)
  61. BL 21 cells (4 replies)
  62. Low level of expression (6 replies)
  63. Thiamine HCl 1M solution? (2 replies)
  64. IP low protein and MS (1 reply)
  65. protein precipitates out during concentrating it (1 reply)
  66. Protein complex immunoprecipitation using Ab/biotin tandem affinity (0 replies)
  67. how to put in evidence protein after IP (2 replies)
  68. expression vector in E. coli (5 replies)
  69. A story of Urea, dialysis and GST affinity purification... hopefully! (1 reply)
  70. In vitro expression extracts (0 replies)
  71. IPTG (2 replies)
  72. Protein lysis buffer (3 replies)
  73. Microcon troubles (4 replies)
  74. Trouble with protein expression in E.coli (2 replies)
  75. What is stringent vector? (2 replies)
  76. expression in minimal media (10 replies)
  77. Elution problens (1 reply)
  78. Protein precipitates out so fast (5 replies)
  79. Concentrating Protein (1 reply)
  80. Insoluble TEV Protease (2 replies)
  81. Endogenous protein purification (2 replies)
  82. Protein in and out of solution (1 reply)
  83. unstable protein expression (1 reply)
  84. GST- protein, batch purification (3 replies)
  85. heterodimer (2 replies)
  86. Nickel IMAC - How to avoid unwanted proteins? (4 replies)
  87. stripped Ni column turn brown yellow (1 reply)
  88. Making Recombinant Proteins (2 replies)
  89. Protein extraction from rat aorta (2 replies)
  90. B-per GST fusion protein purification kit (2 replies)
  91. Protein doesn't bind any column except Ni (10 replies)
  92. Purifying nuclear fraction from skeletal muscle homogenate (1 reply)
  93. Recombinant protein or peptide? (1 reply)
  94. pqe30 (1 reply)
  95. resuspend membrane pellet (2 replies)
  96. Who know anything about dialysis on protein extractions ? (7 replies)
  97. Protein sticks to Ni beads (2 replies)
  98. subcellular fractionation problem (1 reply)
  99. truncation of protein expression (1 reply)
  100. GST fusion protein purification (1 reply)
  101. problems with detecting protein expression (4 replies)
  102. Lysis Buffer for Total Protein Assay (1 reply)
  103. pQE vector protein expression (3 replies)
  104. Which buffer to use? (3 replies)
  105. Elimination of the polyethylenimine (6 replies)
  106. Kozak consensus sequence for insect expression (1 reply)
  107. purification of a basic protein (4 replies)
  108. Sticky Insect cell lysate blocked Ni_NTA column (2 replies)
  109. pgex (5 replies)
  110. stabilization of purified protein solutions (2 replies)
  111. collagen precipitation (2 replies)
  112. Ptn expressed once... and never again!!! (1 reply)
  113. Showed a bigger and broad smear of protein!! (4 replies)
  114. Commercially available bacterial expression system with chaperone coexpression (1 reply)
  115. SWAT for protein expression (1 reply)
  116. Enzyme kinetics (1 reply)
  117. Mammalian protein expression in E. coli (1 reply)
  118. Baculovirus system -swap tag (0 replies)
  119. no expression of my protein (1 reply)
  120. confirming nucleotide state of isolated G protein (1 reply)
  121. purification of Staphylococcus aureus PBP2 (0 replies)
  122. Profinia (0 replies)
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