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BioForum > Methods Discussion > Protein and Proteomics > SDS-PAGE and Western Blotting
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  1. Pinned: Tips on re-use of primary antibody in Western blot (11 replies)
  2. Pinned: Excess acrylamide in wells (24 replies)
  3. Pinned: Multiple antibody blotting - western blot (15 replies)
  4. Pinned: Cutting membrane after transfer (19 replies)
  5. Pinned: PVDF vs. Nitrocellulose for Western blot (12 replies)
  6. Pinned: Old threads on SDS-PAGE and Western Blotting are achived here (0 replies)
  7. Pinned: Western Blotting- A beginner Guide (0 replies)
  8. Protein precipitation by TCA acid + acetone (3 replies)
  9. Non Reducing SDS protein ladder?? (4 replies)
  10. Why do I have to almost "burn" my transfer before I can detect?? (20 replies)
  11. Large protein transfer problem (8 replies)
  12. MEthanol in NC membrane (7 replies)
  13. Does biotinylated proteins will migrate diferently in an SDS than the actual pro (6 replies)
  14. Membrane dried after secondary antibody (6 replies)
  15. Molecular weight marker (13 replies)
  16. Mouse Fibroblasts (4 replies)
  17. How to determine final amount of 20 ng protein per WB well (8 replies)
  18. Secreted protein for loading control (2 replies)
  19. the sensitive amount of protein for western blotting (3 replies)
  20. SDS ladder (3 replies)
  21. Laminin 5 antibody (1 reply)
  22. Pre stained markers (4 replies)
  23. Milk in TBS or TBS-T (4 replies)
  24. Fluorescent visualization of Western blot (2 replies)
  25. SDS-Page issue (5 replies)
  26. problem: doublets and triplets of bands occur in SDS-PAGE (5 replies)
  27. Protein Free Blocking Buffers (1 reply)
  28. Validate SDS-Page (Collagen Typ I) (3 replies)
  29. Problems in runnging Native gel (4 replies)
  30. False positive from Endogenous Antibodies? (1 reply)
  31. Only borders stained in Silver!!! (7 replies)
  32. High Molecular weight protein separation (2 replies)
  33. nonreducing antibody in reducing condition (1 reply)
  34. bromophenol blue (10 replies)
  35. How Best to Clean the Sonicator Tip Between Samples? (3 replies)
  36. MMP Zymography (0 replies)
  37. protein stuck (3 replies)
  38. densitometric analysis for silver stained/coomassie gels? (6 replies)
  39. Quantiying bands. (2 replies)
  40. Native Page transfer/detection problems (1 reply)
  41. nuclear loading control (2 replies)
  42. overloading GAPDH but nice band of interest (2 replies)
  43. Lysis buffer for Immunoprecipitation (2 replies)
  44. Uneven leveling in resolving gel (6 replies)
  45. Could I denature the proteins that were transferred on a membrane? (3 replies)
  46. direct lysis in Laemmli buffer (3 replies)
  47. Too many bands in my SDS-PAGE (5 replies)
  48. Strange lane pattern, yes/no pattern (3 replies)
  49. no front in sds-electrophoresis (1 reply)
  50. Native PAGE w. Western protein marker (4 replies)
  51. small protein blotting (6 replies)
  52. changing colour of front dye in sds-page (7 replies)
  53. SAMPLE MIGRATION PROBLEM IN sds PAGE (9 replies)
  54. Phospatase inhibitors needed for LDS Buffer? (3 replies)
  55. Loading control for nuclear extracts (6 replies)
  56. direct cell lysate (6 replies)
  57. anti-FLAG (M2) cross reaction (6 replies)
  58. What gel thickness and numebr of combs do you use? (1 reply)
  59. Can my sample be going to the buffer instead of the membrane?? (6 replies)
  60. how stable is alpha mouse HRP? (2 replies)
  61. protein precipitation (0 replies)
  62. Uneven bands in SDS-PAGE (5 replies)
  63. Destain after PoneauS (6 replies)
  64. Preparation of tree tissue for western blot (1 reply)
  65. Western blotting question (6 replies)
  66. Acrylamide/Bis Solution for Small Protein (14 kDa) (3 replies)
  67. Can primary antibody be reused ? (6 replies)
  68. Ladder but no protein (4 replies)
  69. Pre-Making your own polyacrylamide gels (4 replies)
  70. why BOIL.. samples for sdspage (9 replies)
  71. pellet became insoluble after boiling. (4 replies)
  72. NFkB western blotting (2 replies)
  73. Mannitol in Lysis Buffer (1 reply)
  74. Detecting Patterns of phosphorylated protein (1 reply)
  75. how long will a 1M DTT solution will last in -20C (5 replies)
  76. Problems with SDS gel (6 replies)
  77. Is possible to generate an anomalous electrical field? (1 reply)
  78. Western Blot of Bacteria (0 replies)
  79. How to solve this western bolt problem? (2 replies)
  80. my membranes are blue! (1 reply)
  81. Sample Diffusion Before Applying Current (4 replies)
  82. Primary Antibody for SPDEF (1 reply)
  83. FLAG Antibody conjugated with FITC (0 replies)
  84. IP and western blot (5 replies)
  85. Blue Native Gel - denaturing proteins after transfer? (3 replies)
  86. Semi-dry transfer conditions (1 reply)
  87. Beginner Question (6 replies)
  88. Nothing appeared at all in my Western! (7 replies)
  89. ImageJ vs. LI-COR Odyssey (1 reply)
  90. Visualizing Small Peptides (16 replies)
  91. nitrocellulose or PVDF (5 replies)
  92. Co-immunopresipitation of Nup 358/RAN BP2(nuclear pore protein) (0 replies)
  93. Variability in GAPDH (5 replies)
  94. crappy bands after developing (3 replies)
  95. cloudy gel (2 replies)
  96. Antibody Characterization (8 replies)
  97. What's the problem with this SDS-PAGE? (8 replies)
  98. ImageJ analysis on IFs (11 replies)
  99. Crazy: ab burnout or something? Higher quant had lower signal? (1 reply)
  100. Antibody Validation (2 replies)
  101. Guanidinium Chloride (11 replies)
  102. Equal loading for Supernatant western blots??? (1 reply)
  103. Primary antibody incubation length (3 replies)
  104. Stripping question (3 replies)
  105. Smears after transfer (2 replies)
  106. 2D SDS-PAGE - wave spots problem (3 replies)
  107. Accidentally Froze Transfer Buffer (1 reply)
  108. antribody gone old? (11 replies)
  109. does 1% SDS prevents proteases? (8 replies)
  110. Ponceau Staining Question (1 reply)
  111. Using NaN3 to quench HRP conjugated secondary Ab (1 reply)
  112. His-tag antibody for western (0 replies)
  113. Beginner Question: Membrane position during Blocking and washing? (1 reply)
  114. bubbles between gel and glass plate (9 replies)
  115. Want to detect oligomers (2 replies)
  116. lost signal (4 replies)
  117. How to extract protein from SDS-PAGE gel? (7 replies)
  118. colorimetry vs chemiluminescence (1 reply)
  119. Yellow Laemmli sample buffer (4 replies)
  120. disappeared signal/blank film (6 replies)
  121. Cast Bis Tris Gel with fibers in the wells (7 replies)
  122. many bands of smaller size than expected (3 replies)
  123. High background.... (6 replies)
  124. Using Photoshop etc on Western bot images (8 replies)
  125. Ghost bands in western blot (7 replies)
  126. how to solve fungus problem on SDS PAGE gel (4 replies)
  127. SDS-PAGE irregular and difuse bands (5 replies)
  128. Unusually viscous samples (2 replies)
  129. SDS page problems (1 reply)
  130. SDS-PAGE Cell lysis problems (3 replies)
  131. freezing after reducing and boiling (10 replies)
  132. Why immunoblotting of actin coming at higher Molec. Weight (1 reply)
  133. proteins stuck at interface between stack and separating gel (2 replies)
  134. Lysyl Oxidase (0 replies)
  135. beginner's Questions on Western Blotting (4 replies)
  136. Lost my proteins ! (3 replies)
  137. Anti-FLAG antibody help (3 replies)
  138. White speckles on western blots (3 replies)
  139. Blocking buffer as antibody dilutent? (4 replies)
  140. How to prepare colloidal coomassie blue? (3 replies)
  141. western blot (17 replies)
  142. protein extraction from tumor samples containing matrigel? (2 replies)
  143. problem with SDS-PAGE (5 replies)
  144. Casp3 (2 replies)
  145. protein quantification by bradford (3 replies)
  146. Amount of protein loaded per well? (4 replies)
  147. prearing gel in advance (6 replies)
  148. caspase3 detection (5 replies)
  149. Invitrogen iBlot (6 replies)
  150. taking protein out of membrane (1 reply)
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