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BioForum > Methods Discussion > Protein and Proteomics > SDS-PAGE and Western Blotting
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  1. Destain after PoneauS (6 replies)
  2. Preparation of tree tissue for western blot (1 reply)
  3. Western blotting question (6 replies)
  4. Acrylamide/Bis Solution for Small Protein (14 kDa) (3 replies)
  5. Can primary antibody be reused ? (6 replies)
  6. Ladder but no protein (4 replies)
  7. Pre-Making your own polyacrylamide gels (4 replies)
  8. why BOIL.. samples for sdspage (9 replies)
  9. pellet became insoluble after boiling. (4 replies)
  10. NFkB western blotting (2 replies)
  11. Mannitol in Lysis Buffer (1 reply)
  12. Detecting Patterns of phosphorylated protein (1 reply)
  13. how long will a 1M DTT solution will last in -20C (5 replies)
  14. Problems with SDS gel (6 replies)
  15. Is possible to generate an anomalous electrical field? (1 reply)
  16. Western Blot of Bacteria (0 replies)
  17. How to solve this western bolt problem? (2 replies)
  18. my membranes are blue! (1 reply)
  19. Sample Diffusion Before Applying Current (4 replies)
  20. Primary Antibody for SPDEF (1 reply)
  21. FLAG Antibody conjugated with FITC (0 replies)
  22. IP and western blot (5 replies)
  23. Blue Native Gel - denaturing proteins after transfer? (3 replies)
  24. Semi-dry transfer conditions (1 reply)
  25. Beginner Question (6 replies)
  26. Nothing appeared at all in my Western! (7 replies)
  27. ImageJ vs. LI-COR Odyssey (1 reply)
  28. Visualizing Small Peptides (16 replies)
  29. Co-immunopresipitation of Nup 358/RAN BP2(nuclear pore protein) (0 replies)
  30. Variability in GAPDH (5 replies)
  31. crappy bands after developing (3 replies)
  32. cloudy gel (2 replies)
  33. Antibody Characterization (8 replies)
  34. What's the problem with this SDS-PAGE? (8 replies)
  35. ImageJ analysis on IFs (11 replies)
  36. Crazy: ab burnout or something? Higher quant had lower signal? (1 reply)
  37. Antibody Validation (2 replies)
  38. Guanidinium Chloride (11 replies)
  39. Equal loading for Supernatant western blots??? (1 reply)
  40. Primary antibody incubation length (3 replies)
  41. Stripping question (3 replies)
  42. Smears after transfer (2 replies)
  43. 2D SDS-PAGE - wave spots problem (3 replies)
  44. Accidentally Froze Transfer Buffer (1 reply)
  45. antribody gone old? (11 replies)
  46. does 1% SDS prevents proteases? (8 replies)
  47. Ponceau Staining Question (1 reply)
  48. Using NaN3 to quench HRP conjugated secondary Ab (1 reply)
  49. His-tag antibody for western (0 replies)
  50. Beginner Question: Membrane position during Blocking and washing? (1 reply)
  51. bubbles between gel and glass plate (9 replies)
  52. Want to detect oligomers (2 replies)
  53. lost signal (4 replies)
  54. How to extract protein from SDS-PAGE gel? (7 replies)
  55. colorimetry vs chemiluminescence (1 reply)
  56. Yellow Laemmli sample buffer (4 replies)
  57. disappeared signal/blank film (6 replies)
  58. Cast Bis Tris Gel with fibers in the wells (7 replies)
  59. many bands of smaller size than expected (3 replies)
  60. High background.... (6 replies)
  61. Using Photoshop etc on Western bot images (8 replies)
  62. how to solve fungus problem on SDS PAGE gel (4 replies)
  63. SDS-PAGE irregular and difuse bands (5 replies)
  64. Unusually viscous samples (2 replies)
  65. SDS page problems (1 reply)
  66. SDS-PAGE Cell lysis problems (3 replies)
  67. freezing after reducing and boiling (10 replies)
  68. Why immunoblotting of actin coming at higher Molec. Weight (1 reply)
  69. proteins stuck at interface between stack and separating gel (2 replies)
  70. Lysyl Oxidase (0 replies)
  71. beginner's Questions on Western Blotting (4 replies)
  72. Lost my proteins ! (3 replies)
  73. Anti-FLAG antibody help (3 replies)
  74. White speckles on western blots (3 replies)
  75. Blocking buffer as antibody dilutent? (4 replies)
  76. western blot (17 replies)
  77. protein extraction from tumor samples containing matrigel? (2 replies)
  78. problem with SDS-PAGE (5 replies)
  79. Casp3 (2 replies)
  80. protein quantification by bradford (3 replies)
  81. Amount of protein loaded per well? (4 replies)
  82. prearing gel in advance (6 replies)
  83. caspase3 detection (5 replies)
  84. Invitrogen iBlot (6 replies)
  85. taking protein out of membrane (1 reply)
  86. Strange pattern after transfer (8 replies)
  87. higher MW non specific band (3 replies)
  88. Even volumes of different samples (3 replies)
  89. Ponceau staining after primary and secondary? (5 replies)
  90. Big white spots on PVDF membrane (1 reply)
  91. A single band on WB smaller than expected (3 replies)
  92. Complete transfer, can it be done or not? (4 replies)
  93. WB does not correspond to protein array (1 reply)
  94. Quantifying Western blots (1 reply)
  95. anti-phosphotyrosine blot on 2mo old samples? (1 reply)
  96. Dot blot (2 replies)
  97. Can add new secondary Ab? (1 reply)
  98. SDS PAGE of Bovine Serum albumin (6 replies)
  99. NATIVE PAGE (1 reply)
  100. Protein much larger on Western blot than predicted (12 replies)
  101. Looking for new endogenous control for WB (3 replies)
  102. wash blots between antibody incubations with TB-'SALINE' pH 7.4 (1 reply)
  103. western blot problems (3 replies)
  104. Recombinant Protein migration (5 replies)
  105. Western Blotting (1 reply)
  106. GST-Tagged protein (4 replies)
  107. western blot gamma interferon (4 replies)
  108. Endogenously Biotinylated Proteins (3 replies)
  109. sds page (1 reply)
  110. Gel mix, acrylamide/bis, Ammonium persulfate, etc disposal (1 reply)
  111. Problem with "smiling effect" on SDS-PAGE gel (11 replies)
  112. Immunofluorescence and Western Blot don't match (5 replies)
  113. protein disapeared from gel! (11 replies)
  114. Western Blot and Odyssey Image Software (1 reply)
  115. big loading control (2 replies)
  116. OMG... (3 replies)
  117. Homemade ECL imaging? (7 replies)
  118. Tris Glycine gel running settings (4 replies)
  119. Ab preabsorption (0 replies)
  120. Custom Antibody (0 replies)
  121. problem with Western blot loadig control (2 replies)
  122. denaturing proteins in gel (1 reply)
  123. western blotting at 37oC? (1 reply)
  124. lysing cells in LSB (2 replies)
  125. Any good software for 2D gel analysis (1 reply)
  126. Markers hardly dissapeared (10 replies)
  127. error in storage temperature of antibodies (4 replies)
  128. Protein extraction from cell culture media (9 replies)
  129. protein band size determination (2 replies)
  130. Could this liver homogenate be used for Western Blot? (2 replies)
  131. Problem with the SDS-PAGE sample prepaeration?!! (6 replies)
  132. Western blot has alot of spots (5 replies)
  133. how to deal with PVDF filter after WB (5 replies)
  134. Problem with running SDS page gel (6 replies)
  135. Problems with large protein (3 replies)
  136. MW marker shows wrong MWs? (8 replies)
  137. Rcovery of proteins from gels (2 replies)
  138. How does the protein fixation on SDS gel work? (3 replies)
  139. lysate buffer (6 replies)
  140. Proteins not running till the end of the gel (2 replies)
  141. Homemade ECL and Amersham HRP secondary (1 reply)
  142. Guanidinium thiocyanate and SDS-PAGE (3 replies)
  143. Is immunoblotting possible after the gel has been silver stained (2 replies)
  144. FISH BLOCK (2 replies)
  145. native PAGE? (2 replies)
  146. Loading Controls for Serum Samples (0 replies)
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