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BioForum > Methods Discussion > PCR, RT-PCR and Real-Time PCR
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  1. Pinned: PCR product purification (28 replies)
  2. Pinned: Important literature for real time PCR users (21 replies)
  3. Pinned: Understanding qPCR and gene expression changes (7 replies)
  4. Pinned: PCR Contamination issues (18 replies)
  5. Pinned: Normalization for RNA or cDNA during two step RT-PCR? (11 replies)
  6. Pinned: How & where to prepare qPCR (4 replies)
  7. Pinned: How to get rid of PCR primer dimer (23 replies)
  8. Pinned: Real-Time RT-PCR one-step and two step issue (9 replies)
  9. Pinned: Primer dimer issue in real time PCR (9 replies)
  10. Pinned: What went wrong in my qPCR? (11 replies)
  11. Blue Juice (2 replies)
  12. Loss of volume in PCR reaction in 96 well plate (3 replies)
  13. primer contamination (3 replies)
  14. Fermentas SYBR Green vs. Qiagen SYBR Green (2 replies)
  15. Single site saturation mutagenesis (0 replies)
  16. Optimal target length (2 replies)
  17. reference genes for qPCR (3 replies)
  18. home made sybr green mix for qpcr (2 replies)
  19. RT-PCR problem (2 replies)
  20. Different types/Brands of BSA? (2 replies)
  21. PCR Efficiency (2 replies)
  22. Confusing bands from PCR (18 replies)
  23. DNA and RNA contamination in RT PCR water controls (2 replies)
  24. Colony PCR Question (6 replies)
  25. 2^-ddCT method for multiple genes (3 replies)
  26. Primer-Blast (3 replies)
  27. primer dimer (2 replies)
  28. qPCR with low efficiency,HELP please!:) (0 replies)
  29. Amplification in NTC and noRT controls (5 replies)
  30. Lost a Band by changing thermocycler (9 replies)
  31. NTC with specific amplification (1 reply)
  32. Primer3 vs Primer BLAST (3 replies)
  33. qPCR using standard curve method (3 replies)
  34. question on molecular beacon (0 replies)
  35. Best time to isolate RNA for qPCR? (1 reply)
  36. Amplifying environmental DNA extract with proofreading enzyme (0 replies)
  37. realtime PCR interpretation-peak found in negative control but no Ct value (6 replies)
  38. Suggestions for optimizing a multiplex PCR? (4 replies)
  39. PCR amplifying 50bp ssDNA ? (6 replies)
  40. asymmetric PCR (1 reply)
  41. Interpreting melting curve data in Sybr Green RT-PCR (12 replies)
  42. CORRECT PCR Incorrect RTPCR (10 replies)
  43. Is there any setting to avoid same base runs in Primer-BLAST? (3 replies)
  44. searching housekeeping genes (4 replies)
  45. Opinion on Eppendorf Realplex qPCR machin (2 replies)
  46. Real time PCR results-interpretation (2 replies)
  47. 2-step or 3-step real time PCR (6 replies)
  48. xChIP qPCR calculation if NoAb ctrl is N.A (1 reply)
  49. Bands appearing out of no where?! (5 replies)
  50. SYBR melting curve vs RT-PCR gel (1 reply)
  51. PCR for cloning (5 replies)
  52. qPCR - no signal detected but plenty of product on gel (3 replies)
  53. primer tm (2 replies)
  54. Suggestions for primer design (2 replies)
  55. Amplify cDNA for qRT-PCR? (4 replies)
  56. Long real-time Amplicon (2 replies)
  57. long DNA amplification (11 replies)
  58. Sybr Green I in real-time PCR reaction (3 replies)
  59. PCR + phusion enzyme = massive errors (4 replies)
  60. Genotyping conditioning-where to start? (1 reply)
  61. RNA from MMA embedded calcified bone (1 reply)
  62. help needed in cDNA synthesis (0 replies)
  63. Whole cell PCR (8 replies)
  64. Real time PCR info (4 replies)
  65. Primer design if sequence is unknown for organism (5 replies)
  66. same primer for reverse transcription and real time (2 replies)
  67. Reference gene qPCR (4 replies)
  68. gDNA pcr product as standard for absolute quantification? (1 reply)
  69. ROX and Rotorgene (2 replies)
  70. QPCR problem! (9 replies)
  71. RNA extraction for qPCR: Trizol vs. column? (5 replies)
  72. relative gene expression with multiple reference genes (1 reply)
  73. pcr trouble solved but I cant seem to understand why (1 reply)
  74. Primers annealing temperatures (8 replies)
  75. Flox and Cre Primers - PCR Troubleshooting (2 replies)
  76. Why is it necessary to add Taq DNA polymerase last during PCR? (8 replies)
  77. Is Taq polymerase still active after staying at 10C for one day? (2 replies)
  78. standard curve (2 replies)
  79. What Factors Affect Ct values of a gene? (1 reply)
  80. E. coli 16s (1 reply)
  81. Mean versus Mediat Ct Values (1 reply)
  82. qRT-PCR no of cycles?? (6 replies)
  83. PCR from BAC (1 reply)
  84. Designing Primers for RT-PCR after ChIP (0 replies)
  85. High Molecular weight artifact LA-PCR 2-step (2 replies)
  86. heating rna before rt reaction? (1 reply)
  87. Which techniques should i do? (2 replies)
  88. roche long range PCR (0 replies)
  89. what primers should I use for DNA sequencing? please help (2 replies)
  90. Megaplex RT Primer Pools: cDNA Synth for Real-Time PCR (0 replies)
  91. Standard curve for RT-PCR (1 reply)
  92. Real-time PCR statistics (0 replies)
  93. how to present rt-pcr results? (0 replies)
  94. Problem amplifying plasmid (1 reply)
  95. high Cp value PCR (2 replies)
  96. no pcr amplification product (9 replies)
  97. Difficulties cloning by PCR (5 replies)
  98. Lowered extension temps with Phusion (6 replies)
  99. question about using random primer for rt-qpcr (0 replies)
  100. PCR Result Explanation (I needed you help) (4 replies)
  101. rna storage at -20 (2 replies)
  102. miScript (Qiagen) - RT of both miRNA and mRNA (1 reply)
  103. Synthesize own Oligo-dT primers (6 replies)
  104. real time PCR data presentation (2 replies)
  105. Lucicferase activity and endogenous transcription (7 replies)
  106. Cell-cycle independant qRT-PCR internal control (1 reply)
  107. reproducability of RT (0 replies)
  108. stats with qPCR ChIP recoveries (0 replies)
  109. 23S rRNA Primer (1 reply)
  110. dNTP or Deoxynucleotide solution Mix (1 reply)
  111. Smearing/ no amplification in PCR (3 replies)
  112. PCR using xt5 (2 replies)
  113. mRNA versus protein expression. Why inverse correlation? (3 replies)
  114. ubiquitary miRNA as positive control (0 replies)
  115. qRTPCR (TaqMan) Data Representation (1 reply)
  116. long range PCR (9 replies)
  117. HEX vs. Cy-5 (3 replies)
  118. Heart ache in real time (1 reply)
  119. Direct PCR (PCR without DNA extraction) (2 replies)
  120. need help on primer calcuation soon (1 reply)
  121. Problems with qRT-PCR (2 replies)
  122. RT-PCR for miRNAs (0 replies)
  123. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! (2 replies)
  124. Primer sets with different annealing temps?? (0 replies)
  125. Primer Dimer in NTC Only? (4 replies)
  126. 2 bands or smear? 2-step RT-PCR (1 reply)
  127. qRT-PCR calculations (2 replies)
  128. Small product amplification problems (4 replies)
  129. Tagged Primers (5 replies)
  130. Two annealing temps? (4 replies)
  131. Homogenizing animal tissue to RT-PCR in real-time (2 replies)
  132. RT-PCR (2 replies)
  133. Multiplex PCR (1 reply)
  134. Freezing primers+SYBR green (2 replies)
  135. Amplfication of fungal DNA (8 replies)
  136. TaqMan qRTPCR amplification oddity (0 replies)
  137. Real-time PCR assay design for prokaryotes (0 replies)
  138. Mysterious PCR Contamination (13 replies)
  139. less steep curve and high Ct value! (2 replies)
  140. PCR problem (4 replies)
  141. Use of iScript RT from Biorad (0 replies)
  142. Gain! (4 replies)
  143. change in ct value due to tube placement (0 replies)
  144. optimal template dna concentrations (1 reply)
  145. Multiplex PCR (1 reply)
  146. low raw fluorescence (0 replies)
  147. Low qPCR efficiency with plasmid template, why? (5 replies)
  148. mRNA level in transgene cell line or knocking down cell line (3 replies)
  149. Question about site directed mutagenesis (1 reply)
  150. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed (3 replies)
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