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BioForum > Methods Discussion > PCR, RT-PCR and Real-Time PCR
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  1. question about using random primer for rt-qpcr (0 replies)
  2. PCR Result Explanation (I needed you help) (4 replies)
  3. rna storage at -20 (2 replies)
  4. miScript (Qiagen) - RT of both miRNA and mRNA (1 reply)
  5. Synthesize own Oligo-dT primers (6 replies)
  6. real time PCR data presentation (2 replies)
  7. Lucicferase activity and endogenous transcription (7 replies)
  8. Cell-cycle independant qRT-PCR internal control (1 reply)
  9. reproducability of RT (0 replies)
  10. stats with qPCR ChIP recoveries (0 replies)
  11. 23S rRNA Primer (1 reply)
  12. dNTP or Deoxynucleotide solution Mix (1 reply)
  13. Smearing/ no amplification in PCR (3 replies)
  14. PCR using xt5 (2 replies)
  15. mRNA versus protein expression. Why inverse correlation? (3 replies)
  16. ubiquitary miRNA as positive control (0 replies)
  17. qRTPCR (TaqMan) Data Representation (1 reply)
  18. long range PCR (9 replies)
  19. HEX vs. Cy-5 (3 replies)
  20. Heart ache in real time (1 reply)
  21. Direct PCR (PCR without DNA extraction) (2 replies)
  22. need help on primer calcuation soon (1 reply)
  23. Problems with qRT-PCR (2 replies)
  24. RT-PCR for miRNAs (0 replies)
  25. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! (2 replies)
  26. Primer sets with different annealing temps?? (0 replies)
  27. Primer Dimer in NTC Only? (4 replies)
  28. 2 bands or smear? 2-step RT-PCR (1 reply)
  29. qRT-PCR calculations (2 replies)
  30. Small product amplification problems (4 replies)
  31. Tagged Primers (5 replies)
  32. Two annealing temps? (4 replies)
  33. Homogenizing animal tissue to RT-PCR in real-time (2 replies)
  34. RT-PCR (2 replies)
  35. Multiplex PCR (1 reply)
  36. Freezing primers+SYBR green (2 replies)
  37. Amplfication of fungal DNA (8 replies)
  38. TaqMan qRTPCR amplification oddity (0 replies)
  39. Real-time PCR assay design for prokaryotes (0 replies)
  40. Mysterious PCR Contamination (13 replies)
  41. less steep curve and high Ct value! (2 replies)
  42. PCR problem (4 replies)
  43. Use of iScript RT from Biorad (0 replies)
  44. Gain! (4 replies)
  45. change in ct value due to tube placement (0 replies)
  46. optimal template dna concentrations (1 reply)
  47. Multiplex PCR (1 reply)
  48. low raw fluorescence (0 replies)
  49. Low qPCR efficiency with plasmid template, why? (5 replies)
  50. mRNA level in transgene cell line or knocking down cell line (3 replies)
  51. Question about site directed mutagenesis (1 reply)
  52. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed (3 replies)
  53. differently behavioring replicates in real-time PCR (1 reply)
  54. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' (4 replies)
  55. Questions about SYBR Green (0 replies)
  56. reconstitution of primers (2 replies)
  57. Different melting curve profiles (0 replies)
  58. Mutagenesis PCR and undesired amplification (2 replies)
  59. pcr doesnt show any band for DNa walking (7 replies)
  60. Endogenous control in bacterial RT-qPCR (4 replies)
  61. Evaporation of samples on the Rotorgene 6000 (0 replies)
  62. Using BLAST to check primers (17 replies)
  63. primer synthesis (0 replies)
  64. Genotyping (6 replies)
  65. dNTPs Quantification? (0 replies)
  66. data acquisition at two temperatures (0 replies)
  67. delta-delta-Ct calibrator (0 replies)
  68. Help Help! Could I use 18S rRNA as internal standard for my RT-PCR rough qu (0 replies)
  69. Syngene GBOX (0 replies)
  70. Why use probes for mtDNA content quantification with RT-PCR (0 replies)
  71. Complicated real-time assay design (0 replies)
  72. Marker migrates slower depending where is loaded (2 replies)
  73. PCR on degraded templates (2 replies)
  74. mRNA level in transgene cell line (0 replies)
  75. PCR help needed (3 replies)
  76. want to purify PCR product from agarose gel but have primer dimer (3 replies)
  77. no bands in my PCR (9 replies)
  78. Tm difference (2 replies)
  79. real time pcr vs sauthern blotting (0 replies)
  80. light bands, dark smear, dark dimers (2 replies)
  81. RT qPCR Newbie, Weird Results (1 reply)
  82. PCR, followed by sequencing... (3 replies)
  83. qPCR and varying cDNA concentrations (1 reply)
  84. Trouble with GAPDH housekeeping gene (3 replies)
  85. PCR program (2 replies)
  86. PCR stopped working!!! HELP!!! (12 replies)
  87. questions about semi-quantitive PCR (2 replies)
  88. Purifying dsDNA from ssDNA after a PCR reaction (3 replies)
  89. Smear problem with my new primer set (2 replies)
  90. REAL TIME PCR 7005 v 2.01 : I need more reference samples! (1 reply)
  91. qPCR and quantity of RNA (2 replies)
  92. about qPCR plates sealing films (4 replies)
  93. qPCR lacks reproduceability (5 replies)
  94. storing large amounts of PCR mastermix beforehand (3 replies)
  95. pcr product quantification (5 replies)
  96. PCR product disappears after restriction digestion (4 replies)
  97. Effects of more cDNA in PCR??? (1 reply)
  98. DNA polymerases for PCR-sequencing (11 replies)
  99. All gradient samples give bands! How come? (4 replies)
  100. PCR product looks less intense compared to others (4 replies)
  101. Genotyping (6 replies)
  102. question about topotaq (0 replies)
  103. Real Time PCR - excluding Ct (2 replies)
  104. Periodic baseline fluctuation in melting curve (0 replies)
  105. sjTREC real time PCR (0 replies)
  106. colony pcr (4 replies)
  107. Real-time PCR Miner (0 replies)
  108. Real Time PCR method comments (2 replies)
  109. truble shooting qiagen 1 step rt pcr kit (2 replies)
  110. probe conc. of Asymmetric PCR (1 reply)
  111. Primers and Taqman Probes mixture question (1 reply)
  112. Normalization of qpcr data (1 reply)
  113. RT-PCR (16 replies)
  114. LC 480 analysis (0 replies)
  115. Making standard dilution for qPCR (1 reply)
  116. platinium taq vs simple taq (1 reply)
  117. Real Time PCR issues (6 replies)
  118. PCR Sample Prep (4 replies)
  119. Gene Expression analysis (4 replies)
  120. 2 Tm peaks with SyBr experiment (6 replies)
  121. How to use Real-time PCR to detect some gene copy numbe in plant genome? (1 reply)
  122. denaturation step of melting surve analysis (2 replies)
  123. contamination (2 replies)
  124. Alternative to netprimer (5 replies)
  125. SYBR and TaqMan (5 replies)
  126. Free software for oligo analysis (3 replies)
  127. way to know if my UNOII pcr machine is working properlly (3 replies)
  128. Freezing DNA have effects on real-time PCR efficiencies (1 reply)
  129. PCR from roots (2 replies)
  130. failed PCR on DNA extract from blood (6 replies)
  131. Interpreting QPCR DATA (3 replies)
  132. can mRNA levels and MFI values be correlated (3 replies)
  133. PCR product stays on agarose gel well !! (11 replies)
  134. RT-PCR inhibitors (0 replies)
  135. PCR Cloning-large primers (4 replies)
  136. RNA isolation for real-time RT-PCR (4 replies)
  137. Is this standard valid? (5 replies)
  138. How can I have same plateau fluorescence? (1 reply)
  139. what does SQ mean? (4 replies)
  140. Real time house keeping genes change (1 reply)
  141. Problem with absolute quatification (1 reply)
  142. PCR contamination with human DNA first and now no bands!!! (4 replies)
  143. Unspecific PCR (7 replies)
  144. PCR data analysis if the efficiencies aren't equal (2 replies)
  145. Preparing PCR reactions from a master mix (3 replies)
  146. Sequencing of scarce real-time amplicons (1 reply)
  147. Will ligase buffer affect polymerase fidelity? (2 replies)
  148. RT-PCR primer dimers and cDNA degradation (2 replies)
  149. Dnase digestion and PCR (4 replies)
  150. expressing data with more than 1 HK gene (2 replies)
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