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BioForum > Methods Discussion > PCR, RT-PCR and Real-Time PCR
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  1. What's the best electronic pipette (Precision) (5 replies)
  2. Relative qPCR dCt analysis (1 reply)
  3. PCR troubleshoooooting (1 reply)
  4. 36Cq limit of detection - why? (0 replies)
  5. extinxtion coefficient ssDNA (0 replies)
  6. restriction digestion of PCR product (7 replies)
  7. Question on wired PCR (1 reply)
  8. Amplification of human genomic DNA (2 replies)
  9. make your own gotaq (2 replies)
  10. reuse pipette tips (2 replies)
  11. β-actin copy numbers (0 replies)
  12. mutant is gone in cDNA (0 replies)
  13. dUTPs in qRTPCR rxn (1 reply)
  14. How to know in which exon a primer match? (1 reply)
  15. Q-PCR: Strange Amplification Curve shape (non exponential) (3 replies)
  16. How to choose the parameters (Tm, cycli) for RT-PCR (1 reply)
  17. trouble amplifying 2.5kb product from genomic DNA (3 replies)
  18. The perfect RT??? which control? (4 replies)
  19. qPCR (5 replies)
  20. ROX utilization (1 reply)
  21. isolation of RNA from insect cells (2 replies)
  22. primer RE over-hang nucleotides (5 replies)
  23. Which the real-time PCR master mix completible to Bio-Rad CXF90 ? (0 replies)
  24. Can I use genomic DNA to make a standard curve for qPCR (5 replies)
  25. Does RT step smplify RNA (2 replies)
  26. good housekeeping for ovary (2 replies)
  27. Problem with primer efficiency analysis (4 replies)
  28. is it necessary to do DNases treatment? (2 replies)
  29. clean up kit for sequencing (0 replies)
  30. Meaning of positive delta G value in hairpin formation (0 replies)
  31. Finding cDNA for making a standard curve for real-time RT-PCR (1 reply)
  32. probe-based qPCR with pico gram gDNA (0 replies)
  33. primer mismatch problem (0 replies)
  34. primer design problem (1 reply)
  35. allele specific pcr (2 replies)
  36. Low 260/230 ratio in my RNA (3 replies)
  37. How to compare different expression levels between genes (1 reply)
  38. PCR unusualband at ~230bps (1 reply)
  39. calibrator (1 reply)
  40. Rotor Gene 3000 and Rotor Gene Q (1 reply)
  41. RT-PCR Help (4 replies)
  42. problem with site directed mutagenesis (4 replies)
  43. minimum amount of cDNA? (6 replies)
  44. Confusing bands from PCR (30 replies)
  45. Problems with GOI Ct's (7 replies)
  46. Settings op a RT-PCR, What is my next step (1 reply)
  47. DMSO or BSA for PCR (6 replies)
  48. slan real time PCR system for validation of microarray results? (1 reply)
  49. primer design (1 reply)
  50. Help me answer this curious student (1 reply)
  51. Absolute quantification (1 reply)
  52. Tris Buffer for PCR reaction (1 reply)
  53. qPCR determination of phage numbers (2 replies)
  54. multiplex PCR (4 replies)
  55. Fast and Furious: Multiplex PCR (10 replies)
  56. PCR a plasmid protein (2 replies)
  57. PCR without thermal cycler? (9 replies)
  58. Realtime PCR machine (2 replies)
  59. PCR set-up calculation nightmares. (3 replies)
  60. Increasing the number of products your PCR produces (3 replies)
  61. Primer desing (2 replies)
  62. RT-PCR problem (4 replies)
  63. Page or HPLC primers (2 replies)
  64. Loss of volume in PCR reaction in 96 well plate (7 replies)
  65. primer-blast from ncbi (3 replies)
  66. Standard curves for PCR efficiency. (2 replies)
  67. HKG validation (2 replies)
  68. Looking for help with my PCR! (3 replies)
  69. Optimal target length (3 replies)
  70. Blue Juice (3 replies)
  71. primer contamination (3 replies)
  72. Fermentas SYBR Green vs. Qiagen SYBR Green (2 replies)
  73. Single site saturation mutagenesis (0 replies)
  74. reference genes for qPCR (3 replies)
  75. home made sybr green mix for qpcr (2 replies)
  76. Different types/Brands of BSA? (2 replies)
  77. PCR Efficiency (2 replies)
  78. DNA and RNA contamination in RT PCR water controls (2 replies)
  79. Colony PCR Question (6 replies)
  80. 2^-ddCT method for multiple genes (3 replies)
  81. Primer-Blast (3 replies)
  82. primer dimer (2 replies)
  83. qPCR with low efficiency,HELP please!:) (0 replies)
  84. Amplification in NTC and noRT controls (5 replies)
  85. Lost a Band by changing thermocycler (9 replies)
  86. NTC with specific amplification (1 reply)
  87. Primer3 vs Primer BLAST (3 replies)
  88. qPCR using standard curve method (3 replies)
  89. question on molecular beacon (0 replies)
  90. Best time to isolate RNA for qPCR? (1 reply)
  91. Amplifying environmental DNA extract with proofreading enzyme (0 replies)
  92. realtime PCR interpretation-peak found in negative control but no Ct value (6 replies)
  93. Suggestions for optimizing a multiplex PCR? (4 replies)
  94. PCR amplifying 50bp ssDNA ? (6 replies)
  95. asymmetric PCR (1 reply)
  96. Interpreting melting curve data in Sybr Green RT-PCR (12 replies)
  97. CORRECT PCR Incorrect RTPCR (10 replies)
  98. Is there any setting to avoid same base runs in Primer-BLAST? (3 replies)
  99. searching housekeeping genes (4 replies)
  100. Opinion on Eppendorf Realplex qPCR machin (2 replies)
  101. Real time PCR results-interpretation (2 replies)
  102. 2-step or 3-step real time PCR (6 replies)
  103. xChIP qPCR calculation if NoAb ctrl is N.A (1 reply)
  104. Bands appearing out of no where?! (5 replies)
  105. SYBR melting curve vs RT-PCR gel (1 reply)
  106. PCR for cloning (5 replies)
  107. qPCR - no signal detected but plenty of product on gel (3 replies)
  108. primer tm (2 replies)
  109. Suggestions for primer design (2 replies)
  110. Amplify cDNA for qRT-PCR? (4 replies)
  111. Long real-time Amplicon (2 replies)
  112. long DNA amplification (11 replies)
  113. Sybr Green I in real-time PCR reaction (3 replies)
  114. PCR + phusion enzyme = massive errors (4 replies)
  115. Genotyping conditioning-where to start? (1 reply)
  116. RNA from MMA embedded calcified bone (1 reply)
  117. Whole cell PCR (8 replies)
  118. Real time PCR info (4 replies)
  119. Primer design if sequence is unknown for organism (5 replies)
  120. same primer for reverse transcription and real time (2 replies)
  121. Reference gene qPCR (4 replies)
  122. gDNA pcr product as standard for absolute quantification? (1 reply)
  123. ROX and Rotorgene (2 replies)
  124. QPCR problem! (9 replies)
  125. RNA extraction for qPCR: Trizol vs. column? (5 replies)
  126. relative gene expression with multiple reference genes (1 reply)
  127. pcr trouble solved but I cant seem to understand why (1 reply)
  128. Primers annealing temperatures (8 replies)
  129. Flox and Cre Primers - PCR Troubleshooting (2 replies)
  130. Why is it necessary to add Taq DNA polymerase last during PCR? (8 replies)
  131. Is Taq polymerase still active after staying at 10C for one day? (2 replies)
  132. standard curve (2 replies)
  133. What Factors Affect Ct values of a gene? (1 reply)
  134. E. coli 16s (1 reply)
  135. Mean versus Mediat Ct Values (1 reply)
  136. qRT-PCR no of cycles?? (6 replies)
  137. PCR from BAC (1 reply)
  138. High Molecular weight artifact LA-PCR 2-step (2 replies)
  139. heating rna before rt reaction? (1 reply)
  140. Which techniques should i do? (2 replies)
  141. roche long range PCR (0 replies)
  142. what primers should I use for DNA sequencing? please help (2 replies)
  143. Megaplex RT Primer Pools: cDNA Synth for Real-Time PCR (0 replies)
  144. Standard curve for RT-PCR (1 reply)
  145. Real-time PCR statistics (0 replies)
  146. how to present rt-pcr results? (0 replies)
  147. Problem amplifying plasmid (1 reply)
  148. high Cp value PCR (2 replies)
  149. no pcr amplification product (9 replies)
  150. Difficulties cloning by PCR (5 replies)
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