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  1. Pinned: Time needed to determine G418 concentration for selection (5 replies)
  2. Pinned: What is the biggest insert for a viral vector? (13 replies)
  3. Pinned: Smear in my restriction digest (11 replies)
  4. Pinned: How to transfect two plasmids in a cell (10 replies)
  5. Pinned: ds-oligo cloning/ligation issues (8 replies)
  6. Pinned: Sequential restriction digestion of plasmid (51 replies)
  7. Pinned: Creating empty vector by self-ligation (30 replies)
  8. Pinned: Problems with Stratagene Site Directed Mutagenesis (21 replies)
  9. Pinned: Help with 3-way ligation (11 replies)
  10. Pinned: Old threads on Molecular Cloning are achived here (0 replies)
  11. Yet another ligation problem (4 replies)
  12. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) (2 replies)
  13. PCR and then ligation (7 replies)
  14. Signal pIgplus vector sequence (0 replies)
  15. 3 way ligation help needed (10 replies)
  16. Promoter Cloning for Luciferase Assay (0 replies)
  17. cloning protocol (1 reply)
  18. Creating mutants (3 replies)
  19. Adeno-x Tet-on vector problem (0 replies)
  20. Ligation Time and Temp (4 replies)
  21. Miniprep (3 replies)
  22. cloning problems in C. elegans expression plasmid (1 reply)
  23. Mammalian expression vector for a partial protein fragment? (0 replies)
  24. help with cloning using pBudce4.1 (0 replies)
  25. Cloning and Expression Method Survey (1 reply)
  26. How to inoculate bacteria? (10 replies)
  27. Cloning woes - Running out of ideas! (6 replies)
  28. Bacteroides mutant construction (0 replies)
  29. What retrovirus vetor can have 7.5kb insert (1 reply)
  30. What fields of research other than microbiology work with bacteria and (1 reply)
  31. Having problem establishing stable cell line...... (5 replies)
  32. Surface expression by tansfection of CHO (3 replies)
  33. need help in my Fse1 restriction ezyme (3 replies)
  34. Heat inactivation/Gel purification or both? (2 replies)
  35. Insert problems with TA cloning (1 reply)
  36. please help!!! with transformation (9 replies)
  37. sequencing primer: T7 or T7promoter? (4 replies)
  38. Best method for Mutagenesis? (2 replies)
  39. problem with subcloning, please help! (8 replies)
  40. Unstable clone? (1 reply)
  41. Restriction enzymes (3 replies)
  42. Failed Transformation of Tuner (DE3) cells (1 reply)
  43. Problems facing in cloning with pBI121 vector (1 reply)
  44. Is my insert present??? (17 replies)
  45. adding koazak sequence to primer (1 reply)
  46. Gel purification of vector DNA - low yield (23 replies)
  47. Need help for restriction enzymes (5 replies)
  48. Mini prep (1 reply)
  49. glycerol stocks (9 replies)
  50. Restriction digest (7 replies)
  51. DNA ligation (2 replies)
  52. cloning faint PCR bands (0 replies)
  53. why sometimes big or small colonies? (16 replies)
  54. Poll: linear vs. supercoiled, blunt vs. overhang? (0 replies)
  55. Moved: doubt in gene annotation (-- replies)
  56. adding C terminal tag to reverse primer (1 reply)
  57. problem in gene cloning (4 replies)
  58. doubts in sequence submission to NCBI (2 replies)
  59. Concentration of DNA for Transformation (1 reply)
  60. Help! Double restriction digestion failed.... (6 replies)
  61. Cloning of enhancer in a vector (0 replies)
  62. Sequence management software (for Mac) (2 replies)
  63. ligation for 24 hrs in 4*C? (3 replies)
  64. Kozak sequences.. (1 reply)
  65. Problems with TA cloning of Fusion PRC product (0 replies)
  66. Creating stable cell lines with pT-Rex-DEST30 (0 replies)
  67. Contruction of Expression Construct (1 reply)
  68. Help! Plasmid disappearing after mini-prep. (3 replies)
  69. Doing mutagenesi for large plasmid or articificial chromosome (1 reply)
  70. Using DNA in gel extract kept at -20ºC (2 replies)
  71. Designing a Plasmid for p53 expression (1 reply)
  72. HELP! Can't double digest pcDNA3.1 vector (6 replies)
  73. Help! No infection with modified vector! (0 replies)
  74. Odd T4 polymerase issue (0 replies)
  75. mutagenesis expert come in~ (11 replies)
  76. t7 promoter (0 replies)
  77. help! looking for vector.... (0 replies)
  78. Help!!! (2 replies)
  79. Help complementing my mutant in trans (1 reply)
  80. Site directed mutagenesis (0 replies)
  81. problem in pcr and purification (3 replies)
  82. DNA yield estimation (1 reply)
  83. Vector NTI from Invitrogen (3 replies)
  84. gfp-tagged protein not expressing- help! (3 replies)
  85. MTTV vs pGEM (0 replies)
  86. transfected too much plasmid (0 replies)
  87. Subcloning (3 replies)
  88. anyone working currently on gateway cloning???? (0 replies)
  89. Dpn1 enzyme (2 replies)
  90. In-fusion advantage PCR cloning kit (12 replies)
  91. High Throughput Microplate Screening (0 replies)
  92. after re-isolation of vector, get single band (4 replies)
  93. Sequencing different colonies (5 replies)
  94. ds-oligos cloning problems (2 replies)
  95. detection of restriction? (1 reply)
  96. Cloning big fragment into smaller vector (3 replies)
  97. Problem with site directed mutagenesis (7 replies)
  98. invitrogen ligase (0 replies)
  99. bacterial stock preparation (3 replies)
  100. COMPETENT CELL PREPARATION (6 replies)
  101. Forgot to add X-gal/IPTG (8 replies)
  102. Problems with Quick change lightning multi site directed mutagenesis kit (10 replies)
  103. Restriction digestion troubleshooting (10 replies)
  104. Subcloning into pMIR-REPORT-luc (6 replies)
  105. Gene expressed in lenti-packaging cells, but not in lenti-infected cells (0 replies)
  106. Making of alkaline lysis solution (4 replies)
  107. Odd point mutations (18 replies)
  108. Ligation/transformation/plates problems (14 replies)
  109. from where can i get the P1 phage (1 reply)
  110. cd44v6 (0 replies)
  111. Which digestion enzyme? (1 reply)
  112. plasmid, BAC, or cosmid? (3 replies)
  113. list of sequence to be added before RE site (3 replies)
  114. multi way ligation or long range pcr? (1 reply)
  115. Ligation of digested plasmid to hairpin oligo (5 replies)
  116. Wierd trouble (3 replies)
  117. Large Molecular weight DNA Extarction (7 replies)
  118. colony PCR (8 replies)
  119. Nucleospin Extract II kit also for plasmids? (5 replies)
  120. insert is missing after TA cloning (6 replies)
  121. Gel electrophoresis and purifying dna (6 replies)
  122. Difficulty cloning vector with multiple Lox sites (2 replies)
  123. T A cloning problems (3 replies)
  124. Random library using TOPO vector (3 replies)
  125. Failed to clone large insert into pcIneo vector.. (0 replies)
  126. Cloning big plasmids / triple ligation (6 replies)
  127. transfection 17kb plasmid in mouse primary cell culture (0 replies)
  128. Vector sequence included in (PCR) amplified insert (2 replies)
  129. Need help: Can't find plasmid in transformed cell!! (0 replies)
  130. Is it pointless to check orientation if non-compatible ends are used? (2 replies)
  131. to construct a new plasmid(in E. coli), what features do i need (1 reply)
  132. generate multiple cloning site in large vector (0 replies)
  133. cDNA library construction (0 replies)
  134. What Carbenecillin concentration does everyone normally use??? (3 replies)
  135. Supercoiling blocking restriction site? (6 replies)
  136. Accidentally deleted enzyme from Vector NTI - how to restore? (1 reply)
  137. What is the maximum size of pUB110? (0 replies)
  138. pCLBABE puro retriviral vector (1 reply)
  139. Maximum recovery time after electroporation (2 replies)
  140. cloning help (9 replies)
  141. Blunt-end cloning problem (6 replies)
  142. Chooseing the best sequence for clonning (6 replies)
  143. [vote] The best Gel extraction kit (11 replies)
  144. ligation troubleshooting (4 replies)
  145. vivo expression technology (25 replies)
  146. Plasmid linearization by PCR (4 replies)
  147. Ligation trouble continues (9 replies)
  148. Cloning a whole gene in pBAD (any comments pls) (4 replies)
  149. can't make the cloning work (3 replies)
  150. Which are the cheap restriction enzymes? (5 replies)
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