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  1. Pinned: online database for antibodies (11 replies)
  2. Pinned: Determine antibody concentration (9 replies)
  3. Pinned: Polyclonal vs. Monoclonal? (8 replies)
  4. Pinned: immunocytochemistry (9 replies)
  5. Pinned: Non-specific binding in ELISA assays (8 replies)
  6. Pinned: DC (Dendritic-Cell) Isolation (19 replies)
  7. Pinned: Immunohistochemistry protocol (4 replies)
  8. Pinned: High background in Western blot (15 replies)
  9. Pinned: mouse splenocyte preparation (5 replies)
  10. Pinned: semi-dry transfer vs wet transfer (5 replies)
  11. Isolation of monocyte from PBMC (2 replies)
  12. Preparing sections from bone (2 replies)
  13. Production of Ascitic Fluid (7 replies)
  14. Immunoblotting (7 replies)
  15. What is anti-cytokine treatment? (1 reply)
  16. Looking for Abtibody Suppliers/Manufacturers (0 replies)
  17. Does anybody have experience in culturing human primary B cells? (0 replies)
  18. co staining problem: same primary species (7 replies)
  19. Staining glutamatergic neurons (0 replies)
  20. His tag antibodies (0 replies)
  21. Monoclonal Antibodies (10 replies)
  22. Companies that synthesize peptide antibodies (3 replies)
  23. Isolation of membrane proteins from Red Blood Cells (0 replies)
  24. IL-1B antibody for immunocytochemistry (0 replies)
  25. Transfection problems (2 replies)
  26. Antisera vs. Antibodies (3 replies)
  27. Lymphocyte Differentiation and Antibiotics (4 replies)
  28. Mixed Leukocyte Reaction (0 replies)
  29. Help needed with IF staining. (2 replies)
  30. How many B cells do I need to get a 1mg of protien (2 replies)
  31. Fc antigen (1 reply)
  32. Desmin antibody: muscle specific? (1 reply)
  33. MHC-II antigens (2 replies)
  34. Why O antigen (ABO blood group) does not activate immune system? (7 replies)
  35. cryosection (4 replies)
  36. may I ask some tips for IEL isolaton? (0 replies)
  37. Mix of alcohol of different molecular weighs? (0 replies)
  38. Th17 Differentiation and Staining (15 replies)
  39. Co-staining Protocol (4 replies)
  40. Immunodepletion of high abundance protein (1 reply)
  41. seperating cells and antibodies (2 replies)
  42. anti tag antibody cross reactivity in mammalian cells (0 replies)
  43. Which DAB for HRP is the best (2 replies)
  44. question: differentiate between B cells (0 replies)
  45. DAB Peroxidase staining (3 replies)
  46. Incubating blood with LPS prior to ELISA (2 replies)
  47. Immunology (5 replies)
  48. collagen staining in cell culture (3 replies)
  49. First time ELIZA? protocol? (5 replies)
  50. PGE conjugation (0 replies)
  51. Sheep Ab (5 replies)
  52. Fluorescence microplate assay (0 replies)
  53. T cell isolation from rat colon (0 replies)
  54. log plot calculation (3 replies)
  55. Dendritic cell infection (2 replies)
  56. DEVD-AFC detection of caspase 3 (0 replies)
  57. ELISA (2 replies)
  58. Fresh Thaw Jurkat -what is the procedure in spinning out dead cells using lympho (1 reply)
  59. cytokine receptor expression on monocytes (1 reply)
  60. antibody differences (1 reply)
  61. Blocking small molecules (1 reply)
  62. Thawing and Plating (2 replies)
  63. macrophage isolation from BM (1 reply)
  64. CD11b clone (3 replies)
  65. prteine cleavage?? (2 replies)
  66. Do RAW264.7 cells express both MHC II alleles? (1 reply)
  67. Fc Blocking in cell cultures (1 reply)
  68. Help with Immunofluorescence staining (0 replies)
  69. 2010 International Symposium on Immune Diseases (0 replies)
  70. sheep red blood cell rosetting protocol (1 reply)
  71. Alcian Blue/Nuc Fast Red - Cryo Sections? (0 replies)
  72. plasma in immunoflourescence (5 replies)
  73. World Congress of Immunodiseases and Therapy (WCIT-2010) (0 replies)
  74. cell based ELISA (0 replies)
  75. OKT3 and anti-CD28 to stimulate Jurkat T-cells (1 reply)
  76. Basic Immunoprecipitation Question (4 replies)
  77. Double labelling in IF (1 reply)
  78. colon immunohistochemistry (5 replies)
  79. nonspecific bands after co-ip (2 replies)
  80. Antibody coupling to Magnetic Beads (3 replies)
  81. Moved: Want to find a penpal (-- replies)
  82. antibody crossreactivity (1 reply)
  83. Need help in choosing rabbit anti-HA tag antibody! (0 replies)
  84. Screening For Lectins in bacteria (4 replies)
  85. BSA Blocking agent (3 replies)
  86. Crosslinking Ab w/Antigen? (2 replies)
  87. How does coculturing work? (5 replies)
  88. please help with Freezing primary cells (1 reply)
  89. ELISA and surface (3 replies)
  90. freezing suspended cells (7 replies)
  91. Complement dependent Cytotoxicity (1 reply)
  92. shipping tissue overseas (0 replies)
  93. Multiplex Cytokine Assays - Antibody coupling (2 replies)
  94. immunosupressive drugs (0 replies)
  95. Mass Spec Identifications of GAPDH (2 replies)
  96. dehydration and rehydration of tissues (1 reply)
  97. moncyte derived macrophage (3 replies)
  98. Immunohistchemistry of red blood cells (1 reply)
  99. Antibody production (1 reply)
  100. MIF protein secretion (2 replies)
  101. Should you keep blood on ice? (2 replies)
  102. How many cells... (2 replies)
  103. How to remove azide from antibody (2 replies)
  104. looking for CD40 ligand-transfected mouse fibroblast L cells (0 replies)
  105. sandwich ELISA (monoclonal and polyclonal matching) (1 reply)
  106. Immunofluorescence problems (0 replies)
  107. Antibody against Bacillus cereus toxins (0 replies)
  108. Culture of NIH3T3-CD40L (4 replies)
  109. antibody labelling (2 replies)
  110. Reduction of non-specific binding (1 reply)
  111. Moved: IP and western blot (-- replies)
  112. Azide interaction with HRP... (5 replies)
  113. Primary antibody and Sodium azide (2 replies)
  114. how to design a protein interaction experiment (4 replies)
  115. Indirect ways to assess opsonophagocytosis (0 replies)
  116. primary antibody not working? (1 reply)
  117. IHC for frozen section for cartilage tissue (1 reply)
  118. THP-1 macrophage autofluroscent (3 replies)
  119. Histology Help (1 reply)
  120. Preabsorption of Ab with Ag still leads to staining in ICC (7 replies)
  121. Manufacturer for IMC-225 (2 replies)
  122. Chimeric antibody Pepsin digestion (1 reply)
  123. Book on Immunocytochemistry (1 reply)
  124. Dendritic cell activation problem (0 replies)
  125. Agarose-conjugated antibody for IP (4 replies)
  126. Immunostaining- opaque layer on fixed cells (4 replies)
  127. Peptide dotblot and ELISA (6 replies)
  128. only monomer immunoprecipitates (2 replies)
  129. Dot Blot question (2 replies)
  130. Dendritic cell immunology (9 replies)
  131. Cells clots forming during preparation mouse splenocytes (4 replies)
  132. mAb purification but precipitated (13 replies)
  133. Non-specific signal in nucleus (1 reply)
  134. Beta Catenin immunofluorescent staining (1 reply)
  135. Anti CD3e (3 replies)
  136. B cell deficient uMT mice and Abs (0 replies)
  137. Help with unwanted fluorescent lines in sections (12 replies)
  138. Cryosection problem (2 replies)
  139. difference between IHC and ICC (3 replies)
  140. Weak signal in WESTERN BLOT (1 reply)
  141. YFP staining (2 replies)
  142. Problems with non-specific nuclei staining (0 replies)
  143. Anti-Flag (M2) agarose beads (0 replies)
  144. antibody interaction (1 reply)
  145. HbA1c antibody (0 replies)
  146. Gamma-h2ax foci HELP!!!!!!!!!! (0 replies)
  147. Immune Sytem Assignment Assessment (0 replies)
  148. Antibody titration (5 replies)
  149. inliximab sequence (5 replies)
  150. Granzyme B (0 replies)
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