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General Lab Techniques
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General Lab Techniques
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Pinned:
Useful Dilution Techniques & Calculations
(10 replies)
Pinned:
Being good at the bench
(4 replies)
Pinned:
Agarose gel electrophoresis troubleshooting
(12 replies)
Pinned:
Tips on ethanol wash after nucleic acid precipitation
(20 replies)
Pinned:
How to sterilize cover slips (glasses)?
(27 replies)
Pinned:
How to keep articles organized
(10 replies)
Pinned:
Liquid and solid autoclave
(15 replies)
Pinned:
How do you mix PCR reactions - swish, flick or nothing?
(14 replies)
Pinned:
Best storage temperature for samples - serum, protein, tissue, DNA, RNA
(4 replies)
Pinned:
Convert g into rpm
(3 replies)
Pinned:
Centrifuge
(6 replies)
Pinned:
How do you prepare PCR stock solution to minimizing pipetting
(5 replies)
High Throughput Microplate Screening
(3 replies)
Pyrogen
(1 reply)
EDTA as an anticoagulant
(2 replies)
Treatments to clean silanized glass tubes
(2 replies)
Autoclave PBS-A with Na2HPO4 = cloudy?
(1 reply)
My acrylimide gel rips.
(3 replies)
Autoclaved water with white flecks in it?
(11 replies)
Measurement of phenol red
(1 reply)
Exctinction Co-efficient
(3 replies)
removing fibrinogen from serum
(1 reply)
protein concentration
(3 replies)
Northern blotting versus southern blotting
(3 replies)
qestion about sequencing
(2 replies)
how to remove cover slip of old slide
(1 reply)
How to calculate GI50
(1 reply)
5M NaF won't dissolve!
(7 replies)
MPN tables
(0 replies)
A260/A280 at 1.3
(14 replies)
Immunofluorescence
(3 replies)
concentrating RNA samples
(7 replies)
Making TE buffer for RNA disolution
(4 replies)
mammalian promoter
(1 reply)
Fluorometer vs spectrophotometer
(5 replies)
western
(1 reply)
cell concentrations
(2 replies)
PBS and blocking buffer for ELISA applications
(1 reply)
DNA Ladder Issues
(5 replies)
why gel is not running in straight line??
(13 replies)
labware for a new lab
(1 reply)
0.15M Borate Buffer pH 8.3
(3 replies)
Accuracy???!!!
(5 replies)
Injection marker
(0 replies)
Variation between spectros
(1 reply)
Differences among Phosphate buffer, Tris-HCl and Tris Buffer
(1 reply)
dna concentration
(5 replies)
Preparation of HBr
(1 reply)
pHmeter calibratio
(3 replies)
Large Circular DNA
(1 reply)
Compound Screening
(1 reply)
magnetic beads
(4 replies)
MYCOPLASMOLOGY - GC/CFU
(0 replies)
MYCOPLASMA STUDIES
(0 replies)
Tris Buffer Problem
(4 replies)
Acrylamide vortexing!!!
(7 replies)
Help me!!!
(0 replies)
pH of Urea and Acrylamide solutions!!!
(4 replies)
Problems with an Osmometer
(1 reply)
Difference between EDTA and HEDTA
(4 replies)
Cell Invasion Assay
(5 replies)
Acrylamide Disposal!!!
(10 replies)
DNA extraction from semen straws
(0 replies)
Tricky buffer preparation
(1 reply)
casting agarose gel troubleshooting
(8 replies)
Luminometers with and without injectors
(0 replies)
Extraction of genomic bacterial DNA from Lichen samples
(0 replies)
Intra-assay reproducibility
(2 replies)
Catalog numbers for coverslips and glass slides
(4 replies)
How to weigh micrograms
(2 replies)
Mass spectrometry analysis
(0 replies)
"Flies Attack" in Lab
(15 replies)
staining agarose and gelatin
(0 replies)
endotoxin amount defined as EU/ml
(5 replies)
Rescue my DNA extraction
(1 reply)
Pulse Field Gel Electrophoresis..smearing of bands
(0 replies)
SDS-PAGE sample loading problem
(0 replies)
Optimal stock concentration for gDNA storage
(1 reply)
Digestion Protocols
(1 reply)
Pimp my Biosafety Hood!
(8 replies)
Tips for using fluorescently labeled DNA primers
(0 replies)
Where to order FOA?
(1 reply)
platelet isolation
(0 replies)
reconstitution of lyophilized enzyme (soybean 15-lipoxygenase-1)
(2 replies)
Standard Curve for OD Turbidity vs Cell density
(6 replies)
In Vitro Toxicity Testing
(0 replies)
Transport of miniprep
(2 replies)
How dangerous is EtBr?
(9 replies)
pH meter
(1 reply)
Use of 70% ethanol to remove RNAse from working area
(2 replies)
b-complex and ascorbic acid extraction
(0 replies)
primer design
(1 reply)
need help
(1 reply)
could humic acid dissolve in 70% alcohol
(2 replies)
LB-Cm Agar plates
(2 replies)
Zinc chloride calculation
(2 replies)
Detach and resuspend after centrifugation
(0 replies)
Hydrogen Peroxide (H2O2) Generator
(1 reply)
Best way to resuspend E. coli?
(7 replies)
Cell concentration Question
(5 replies)
Dead Dendritic cells ... please help
(1 reply)
questions about enzymatic acitivity assay
(4 replies)
autoclaving
(27 replies)
Water
(1 reply)
Nuclease contamination or what???
(2 replies)
Multicomponent Buffers
(0 replies)
Moisture in Agar plates!
(3 replies)
Problem transferring HIV-1 tat protein to a PVDF membrane for western blot
(5 replies)
simple calculation
(4 replies)
Imaging Software
(1 reply)
Protocol book
(4 replies)
Size of pores in polyacrylamide gel
(0 replies)
260/280 and 260/230 readings...
(6 replies)
cell count
(3 replies)
homogenization devices
(1 reply)
DNA ladders on a northern blot
(1 reply)
%w/v question
(2 replies)
increase the amount of cDNA
(3 replies)
Frozen Pertussis Toxin?
(1 reply)
problems about cryosection
(0 replies)
assay for penicillinase
(1 reply)
Protein Concentration assay (Bradford assay)
(2 replies)
sodium phosphate buffer recipe
(2 replies)
Help plz~ white mass in the mouse abdomen days after i.p. of tamoxifen
(2 replies)
Purification of virus particles
(2 replies)
gus vaccum infiltration
(0 replies)
how to prepare RNAse A from powdered form
(1 reply)
Outer membrane preparation protocol.
(0 replies)
Shelf life of buffers? (unopened)
(1 reply)
nylon mesh
(4 replies)
Determining ideal page conditions
(5 replies)
Measuring OD for a bacteria culture
(7 replies)
Look for Basic HPLC Book (PDF Format)
(2 replies)
Any experience with environmental S.E.M.?
(2 replies)
cell cryosection
(5 replies)
Photography DIGITAL CAMERA FOR MICROSCOPE
(0 replies)
Virus Enumeration Problems
(0 replies)
Problems with pH readings all over the place
(3 replies)
A260/230 readings post gel extraction
(17 replies)
HPLC -problem with solvent peak
(20 replies)
Optimizing the ISRE-SEAP assay
(1 reply)
Preparing Methyl Cellulose
(4 replies)
HELP! I have big trouble with ghost peak!
(1 reply)
DNAdynamo
(2 replies)
Blue Juice loading dye & Gel extraction kit
(6 replies)
Agarose gel with EtBr exposed to light over night.
(3 replies)
Moved:
Neutrophil separation
(-- replies)
Fixation of immune cells
(2 replies)
measuring nucleic acid concentration on nanodrop
(8 replies)
chitinase assay
(1 reply)
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