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Full Version: siRNA, microRNA and RNAi
BioForum > Methods Discussion > siRNA, microRNA and RNAi
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  1. Pinned: siRNA target selection problem (7 replies)
  2. Pinned: Different siRNA transfection agents (7 replies)
  3. Pinned: Old threads on "siRNA, microRNA and RNAi" are achived here (1 reply)
  4. Pinned: Promoters and vectors for shRNA? The need for specific vectors and promoters? (4 replies)
  5. Pinned: olligo annealing for shRNA cloning (17 replies)
  6. Pinned: stable transfection with siRNA expression vector (4 replies)
  7. Pinned: Nuclear RNA knockdown with siRNA (3 replies)
  8. Pinned: RNA Interference (7 replies)
  9. miRNA gene UTRs? (3 replies)
  10. Cos7 Cells to produce retrovirus (0 replies)
  11. Verified RNAi Sequences (1 reply)
  12. Transfection Efficacy (2 replies)
  13. Question about miRNA-star and Taqman Q-PCR miRNA array validation (0 replies)
  14. siRNA and plasmid cotransfection (1 reply)
  15. rna from plasmid (1 reply)
  16. in vitro siRNA (2 replies)
  17. How to batch search my microRNA accession numbers? (1 reply)
  18. SiRNA- gene silencing in brain Organotypic slice cultures (3 replies)
  19. shRNA stable line (8 replies)
  20. T cell transduction (3 replies)
  21. design parameters for multiple siRNA expression in vector (4 replies)
  22. retrovirus shRNA (0 replies)
  23. Co-transfection of miRNA and vector expressing target sequence (2 replies)
  24. microRNA AAAS webinar (0 replies)
  25. Pronounciation of miRNA (2 replies)
  26. miRNA TARGETS (4 replies)
  27. Who are the big names in miRNA research? (4 replies)
  28. 3'UTR constructs (1 reply)
  29. miScript (Qiagen) - RT of both miRNA and mRNA (4 replies)
  30. How to overexpress star miRNA (miRNA*) from pre-miRNA construct (4 replies)
  31. Is my primer going to work for qRT-PCR? (5 replies)
  32. Ncode qRT-PCR (4 replies)
  33. Concatemerization of miRNA... (1 reply)
  34. To Fizban (9 replies)
  35. is dsRNA stable in cells? (0 replies)
  36. ShRNA vectors for other than mammalian systems (6 replies)
  37. mirVana kit (5 replies)
  38. MiRna (2 replies)
  39. microRNAs (1 reply)
  40. miRNA isolation from liquid samples (2 replies)
  41. siRNA with low mRNA levels (7 replies)
  42. mature star miRNA (2 replies)
  43. shRNA plasmid (2 replies)
  44. ? degenerate base codes, e.g., D = U, G, A (1 reply)
  45. Inhibition of miRNA doesn't enhance its target expression (4 replies)
  46. shRNA shows knockdown in protein expression but no change in RT-PCR (2 replies)
  47. Can Oligofectamine be used in this way? (0 replies)
  48. Ncode miRNA RT & qPCR system (3 replies)
  49. ABI Taqman Low density microRNA Array card (3 replies)
  50. si-Fi, Software for RNAi off-target prediction (0 replies)
  51. Cells to Ct RNA isolation kit (Ambion) (0 replies)
  52. how to test if a given microRNA regulates a gene of interest (7 replies)
  53. look for E.coli DB3.1 bacteria (2 replies)
  54. miR family (1 reply)
  55. RT-PCR assay on mature miRNAs using RotorGene machine (0 replies)
  56. miRcheck (0 replies)
  57. miR family (0 replies)
  58. T-DNA multiple copy number and RNAi (0 replies)
  59. T-DNA multiple copy number and RNAi (0 replies)
  60. novel microRNA prediction (2 replies)
  61. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin (4 replies)
  62. pri-miR transcript (1 reply)
  63. small RNA Northern (1 reply)
  64. siRNA electroporation (0 replies)
  65. MCF-7 cells dying after siRNA transfection + estrogen (1 reply)
  66. Is it possible to reuse electroporation cuvette to transfect siRNA (4 replies)
  67. microRNAs (2 replies)
  68. translation of cleaved miRNA targets (3 replies)
  69. Problems with gene knockdown (6 replies)
  70. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL (1 reply)
  71. Hammerhead ribozyme cleavage (2 replies)
  72. Silencer® GFP (eGFP) siRNA – AM4626 (2 replies)
  73. Transfection of siRNA into activated T cells (0 replies)
  74. siRNA on primary DRG cell (3 replies)
  75. HepG2 siRNA transfection using Nucleofector (0 replies)
  76. miR-145 and miR-143 (0 replies)
  77. siRNA (2 replies)
  78. prediction of novel microRNAs from deep sequencing data? (1 reply)
  79. ready made or customize miRNA microarray chip (2 replies)
  80. RNAi (1 reply)
  81. pcDNA/pSUPER (1 reply)
  82. in vitro pri-mirna processing assays protocol (0 replies)
  83. shRNA stable lines (3 replies)
  84. p53 and miRNA (0 replies)
  85. Is it Possible (1 reply)
  86. annotated 3'utr? (1 reply)
  87. Max. length for 3'UTR in luciferase assay? (1 reply)
  88. target protector and site mutagenesis (6 replies)
  89. Complementation experiment (4 replies)
  90. miRNA mimics (2 replies)
  91. Why the fluoresence is dim or dispear in miRNA vector? (3 replies)
  92. In vitro shRNA screening trouble (4 replies)
  93. why using siRNA knock down,but get the mRNA products? (3 replies)
  94. about the microRNA seqence database (3 replies)
  95. deep sequencing miRNA (1 reply)
  96. Lamin A/C Control (0 replies)
  97. Designing new RNAi (2 replies)
  98. Accell siRAN from Dharmacon (1 reply)
  99. HIV and miRNA (0 replies)
  100. miRNA Arrays - which to use? (2 replies)
  101. Got miRNA, need targets? (1 reply)
  102. siRNA modulating alternative splicing (0 replies)
  103. New to shRNA (4 replies)
  104. Knockdown of miRNAs (3 replies)
  105. Mir34a positive control cells (4 replies)
  106. small RNA cDNA library (0 replies)
  107. endogenous miRNA of cos-7 cell (2 replies)
  108. Controls for Overexpressing miRNAs (1 reply)
  109. Two new citations involving miRNA suppression (0 replies)
  110. miRNA real time PCR by SYBR green methods (1 reply)
  111. cloning miRNA library (4 replies)
  112. Can shRNA be transfected without vector...?? (4 replies)
  113. Which shRNA provider or calculator do YOU use? (3 replies)
  114. Silencing Viral MicroRNA as a Novel Antiviral Therapy? (0 replies)
  115. KSRP binding to loop regions of miRNA: maturation regulation (0 replies)
  116. Qiagen miRNeasy kit (2 replies)
  117. Quantify RNAi(siRNA) efficacy (2 replies)
  118. about the total RNA isolation (trizol methods) and further Dnase I (2 replies)
  119. Ask for help about the miRNA qRT-PCR by SYBR green methods (0 replies)
  120. Denaturing the human RISC complex (0 replies)
  121. So confused with RNAi (3 replies)
  122. Lenti viral Vector with GFP (4 replies)
  123. Probe size for miRNA Northern blot? (8 replies)
  124. inducible shRNA vector system (6 replies)
  125. Citation: MicroRNA-125b is a novel negative regulator of p53 (0 replies)
  126. Moved: How to concentrate lenti or retroviral particules (-- replies)
  127. miRNA Northern Blot gel (4 replies)
  128. Transcription reaction (1 reply)
  129. To Fizban: miRNA validation (5 replies)
  130. Cloning multiple miR30 based shRNAs into a Lenti (2 replies)
  131. How many bp should be left at 5' and 3' flanking regions needed for miRN (4 replies)
  132. shRNA from SIGMA (5 replies)
  133. RNAi in Zebrafish (4 replies)
  134. How to design probes for microRNA Northern Blot (4 replies)
  135. Could I use transcriptomics data to look for pri-miRNA sequence (2 replies)
  136. siRNA concentration in divinding-growing cells (2 replies)
  137. siRNA transfection of RS4-11 and MV-4-11 Cells (1 reply)
  138. miRNA turnover rate (1 reply)
  139. siRNA resistant gene (4 replies)
  140. siRNA cell function examples (1 reply)
  141. shRNA transgenic mice (1 reply)
  142. Good SiRNA Gene silencing (1 reply)
  143. ? definition of: thermodynamic stability at the 5' end (2 replies)
  144. cellular siRNA localization (4 replies)
  145. Moved: meetings (-- replies)
  146. m RNA5'cap (0 replies)
  147. workflow - start from microarray (4 replies)
  148. Define Trasnsfection efficiency interms of siRNA experiment (12 replies)
  149. Amaxa siRNA (3 replies)
  150. Dicer-KO (4 replies)
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