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Full Version: siRNA, microRNA and RNAi
BioForum > Methods Discussion > siRNA, microRNA and RNAi
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  1. Pinned: pre-miRNA existence (11 replies)
  2. Pinned: olligo annealing for shRNA cloning (18 replies)
  3. Pinned: siRNA target selection problem (7 replies)
  4. Pinned: Different siRNA transfection agents (7 replies)
  5. Pinned: Old threads on "siRNA, microRNA and RNAi" are achived here (1 reply)
  6. Pinned: Promoters and vectors for shRNA? The need for specific vectors and promoters? (4 replies)
  7. Pinned: stable transfection with siRNA expression vector (4 replies)
  8. Pinned: Nuclear RNA knockdown with siRNA (3 replies)
  9. Pinned: RNA Interference (7 replies)
  10. qrt-pcr and northern blot ,which way is better? (4 replies)
  11. How to estimate viral titre? (1 reply)
  12. Effect of MOI on lentiviral shRNA knockdown (1 reply)
  13. total RNA (for miRNA) extraction from human serum (11 replies)
  14. Detecting small RNAs via DeepSeq? (0 replies)
  15. microRNAs (3 replies)
  16. miRNA isolation (3 replies)
  17. Specific targeting of alternative splice forms? (1 reply)
  18. siRNA againts Beta Actin Help PLease (0 replies)
  19. Review of zebrafish miRNA (1 reply)
  20. If we somehow downregulate pri-miRNA, mature miRNA will also be downregulated? (0 replies)
  21. To Fizban (12 replies)
  22. Who are the big names in miRNA research? (5 replies)
  23. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m (6 replies)
  24. Assessing Pre-miR transfection efficiency (0 replies)
  25. Help with multiplexing! (3 replies)
  26. Why there is no knockdown? (3 replies)
  27. microRNAs and mRNAs. Interpreting gene expression profiles (4 replies)
  28. Down-regulated anti-sense miRNA strand following pre-miR overexpression (0 replies)
  29. about the total RNA isolation (trizol methods) and further Dnase I (3 replies)
  30. Bands in a miRNA gel (0 replies)
  31. miRNA isolation from liquid samples (3 replies)
  32. about pre-miRNA cloned into lentivirus vectors (4 replies)
  33. si-Fi, Software for RNAi off-target prediction (1 reply)
  34. Optimizing transfection of miRNA (2 replies)
  35. Multiplexing miRNA RT for QPCR (0 replies)
  36. knockdown issue...please help... (1 reply)
  37. pRL-TK as primary Vector ? (1 reply)
  38. Cell line purchase for siRNA (0 replies)
  39. Tool to search for motifs within microRNAs? (5 replies)
  40. Weird No RT control problem in the precursor miRNA experiment (0 replies)
  41. Half-life of Transfected pre-miRNA and miRNA inhibitors (2 replies)
  42. miRNA promoter (7 replies)
  43. Cells to Ct RNA isolation kit (Ambion) (1 reply)
  44. MirVana + RNAlater white pellet? (3 replies)
  45. iFect reagent in vivo (2 replies)
  46. Transfection of PolyI:C using Lipofectamine (0 replies)
  47. Lentivial Knockdown of miRNAs (3 replies)
  48. Trouble transfecting bend.3 cells (0 replies)
  49. LNA probes - in vivo vs in vitro (1 reply)
  50. Can Oligofectamine be used in this way? (1 reply)
  51. Question about the control for an RNAi Experiment (3 replies)
  52. siRNA staining? (1 reply)
  53. FUNGI SILENCING (2 replies)
  54. Knockdown miRNA in a breast cancer cell line (0 replies)
  55. microRNA database (1 reply)
  56. Sample Pooling (1 reply)
  57. how do I know if there is a miRNA modulating expression? (3 replies)
  58. How to design the 3UTR seq for luciferease Assay (1 reply)
  59. shRNA selection in suspension cell culture (6 replies)
  60. How to get pri-microRNA sequence of plants (1 reply)
  61. shRNA negative control (2 replies)
  62. retrovirus shRNA (1 reply)
  63. confirming an siRNA gene knockdown using qRT-PCR (1 reply)
  64. question on pri-miRNA (0 replies)
  65. One miRNA modulating expression of about 600 genes (1 reply)
  66. Verified RNAi Sequences (4 replies)
  67. Cos7 Cells to produce retrovirus (2 replies)
  68. How to batch search my microRNA accession numbers? (2 replies)
  69. miRNA gene UTRs? (3 replies)
  70. Transfection Efficacy (2 replies)
  71. Question about miRNA-star and Taqman Q-PCR miRNA array validation (0 replies)
  72. siRNA and plasmid cotransfection (1 reply)
  73. rna from plasmid (1 reply)
  74. in vitro siRNA (2 replies)
  75. SiRNA- gene silencing in brain Organotypic slice cultures (3 replies)
  76. shRNA stable line (8 replies)
  77. T cell transduction (3 replies)
  78. design parameters for multiple siRNA expression in vector (4 replies)
  79. Co-transfection of miRNA and vector expressing target sequence (2 replies)
  80. microRNA AAAS webinar (0 replies)
  81. Pronounciation of miRNA (2 replies)
  82. miRNA TARGETS (4 replies)
  83. 3'UTR constructs (1 reply)
  84. miScript (Qiagen) - RT of both miRNA and mRNA (4 replies)
  85. How to overexpress star miRNA (miRNA*) from pre-miRNA construct (4 replies)
  86. Is my primer going to work for qRT-PCR? (5 replies)
  87. Ncode qRT-PCR (4 replies)
  88. Concatemerization of miRNA... (1 reply)
  89. is dsRNA stable in cells? (0 replies)
  90. ShRNA vectors for other than mammalian systems (6 replies)
  91. mirVana kit (5 replies)
  92. MiRna (2 replies)
  93. microRNAs (1 reply)
  94. siRNA with low mRNA levels (7 replies)
  95. mature star miRNA (2 replies)
  96. shRNA plasmid (2 replies)
  97. ? degenerate base codes, e.g., D = U, G, A (1 reply)
  98. Inhibition of miRNA doesn't enhance its target expression (4 replies)
  99. shRNA shows knockdown in protein expression but no change in RT-PCR (2 replies)
  100. Ncode miRNA RT & qPCR system (3 replies)
  101. ABI Taqman Low density microRNA Array card (3 replies)
  102. how to test if a given microRNA regulates a gene of interest (7 replies)
  103. look for E.coli DB3.1 bacteria (2 replies)
  104. miR family (1 reply)
  105. RT-PCR assay on mature miRNAs using RotorGene machine (0 replies)
  106. miRcheck (0 replies)
  107. miR family (0 replies)
  108. T-DNA multiple copy number and RNAi (0 replies)
  109. T-DNA multiple copy number and RNAi (0 replies)
  110. novel microRNA prediction (2 replies)
  111. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin (4 replies)
  112. pri-miR transcript (1 reply)
  113. small RNA Northern (1 reply)
  114. siRNA electroporation (0 replies)
  115. MCF-7 cells dying after siRNA transfection + estrogen (1 reply)
  116. Is it possible to reuse electroporation cuvette to transfect siRNA (4 replies)
  117. translation of cleaved miRNA targets (3 replies)
  118. Problems with gene knockdown (6 replies)
  119. Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL (1 reply)
  120. Hammerhead ribozyme cleavage (2 replies)
  121. Silencer® GFP (eGFP) siRNA – AM4626 (2 replies)
  122. Transfection of siRNA into activated T cells (0 replies)
  123. siRNA on primary DRG cell (3 replies)
  124. HepG2 siRNA transfection using Nucleofector (0 replies)
  125. miR-145 and miR-143 (0 replies)
  126. siRNA (2 replies)
  127. prediction of novel microRNAs from deep sequencing data? (1 reply)
  128. ready made or customize miRNA microarray chip (2 replies)
  129. RNAi (1 reply)
  130. pcDNA/pSUPER (1 reply)
  131. in vitro pri-mirna processing assays protocol (0 replies)
  132. shRNA stable lines (3 replies)
  133. p53 and miRNA (0 replies)
  134. Is it Possible (1 reply)
  135. annotated 3'utr? (1 reply)
  136. Max. length for 3'UTR in luciferase assay? (1 reply)
  137. target protector and site mutagenesis (6 replies)
  138. Complementation experiment (4 replies)
  139. miRNA mimics (2 replies)
  140. Why the fluoresence is dim or dispear in miRNA vector? (3 replies)
  141. In vitro shRNA screening trouble (4 replies)
  142. why using siRNA knock down,but get the mRNA products? (3 replies)
  143. about the microRNA seqence database (3 replies)
  144. deep sequencing miRNA (1 reply)
  145. Lamin A/C Control (0 replies)
  146. Designing new RNAi (2 replies)
  147. Accell siRAN from Dharmacon (1 reply)
  148. HIV and miRNA (0 replies)
  149. miRNA Arrays - which to use? (2 replies)
  150. Got miRNA, need targets? (1 reply)
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