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  1. Serum Batch Test.... (3 replies)
  2. Ca2+ imaging (0 replies)
  3. what is the activity of TAQ at 60 centigrade? (2 replies)
  4. no 18S band on RNA agarose gel (1 reply)
  5. b-mercaptoethanol in lysis buffer (1 reply)
  6. Could You Suggest me A good KIt for DNA extraction from Yeast? (1 reply)
  7. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh (3 replies)
  8. Plasmid isolation, help with dilution step? (7 replies)
  9. nylon membranes bleaching (2 replies)
  10. Site directed mutagenesis (4 replies)
  11. Extraction of large size genomic DNA (3 replies)
  12. RNA in situ hybridization (0 replies)
  13. DNA vs Protein Stains (1 reply)
  14. multiple bands in my RNA sample? (2 replies)
  15. Moved: Please anybody can explain the cloning related to plant vector construction (-- replies)
  16. Incorporate biotinylated-dNTPs by a polymerase (1 reply)
  17. full digestion of plasmid to get only dNTPs (3 replies)
  18. Moved: Creating empty vector (-- replies)
  19. splice variant encoding same protein (1 reply)
  20. expession only in +ve controls (4 replies)
  21. wrong gene (13 replies)
  22. Moved: Problems with qRT-PCR (-- replies)
  23. Why hasnt HIF-1A gone up during hypoxia? (3 replies)
  24. minimum length for the gene to be amplified in PCR (6 replies)
  25. DNA Question for TV Show (4 replies)
  26. primers for mRNA probe synthesis (0 replies)
  27. 5' RACE products appearance on 1.5% agarose gel (8 replies)
  28. dNTP Quantity (3 replies)
  29. gene design (2 replies)
  30. Amplification dwindles using little template RT-PCR (3 replies)
  31. Moved: Molecular Biologist Salary Range (-- replies)
  32. Phenol chloroform extraction of DNA (3 replies)
  33. How to get recombined Tcr sequence?likeMOG ,OVA (0 replies)
  34. ligation using PEG 6000 and subsequent reactions (0 replies)
  35. RNA isolation form plants (4 replies)
  36. RNA from Sheep and Bovine blood (3 replies)
  37. Degenerate PCR Size Limit? (3 replies)
  38. help with transformation! thanks!! (15 replies)
  39. Number of genes and number of proteins in humans (1 reply)
  40. How to increase the concentration insert or vector or no cut plasmid (4 replies)
  41. Ligation confusion (0 replies)
  42. 5' end RNA biotin labeling (2 replies)
  43. RNA isolation from murine pancreas (2 replies)
  44. Yeast complementation test (1 reply)
  45. Digestion of a plasmid (1 reply)
  46. small PCR products on agarose gel? (8 replies)
  47. Heating RNA samples for electrophoresis (3 replies)
  48. latarcin-1 (1 reply)
  49. Removing phenol contamination from total RNA (5 replies)
  50. Bone Marrow Growth Supplement (2 replies)
  51. SaOS2 Cell Line and P57 (0 replies)
  52. suggestions on the genomic DNA quality (2 replies)
  53. Maxi Prep cDNA contamination (2 replies)
  54. How to check antibodies for viruses (1 reply)
  55. Genotyping MHC alleles in mice (0 replies)
  56. Gel Electrophoresis Interpretation (3 replies)
  57. VSVG-Retroviral Production Problem (1 reply)
  58. Moved: Can my results go in publication (-- replies)
  59. transfection (1 reply)
  60. cDNA question (3 replies)
  61. finding sequence to IL21 (9 replies)
  62. Sequencing very short (60-80bp) amplicons ? (3 replies)
  63. Toxic Plasmid?? Toxic Insert?? (2 replies)
  64. low viral load in HIV positive sample (1 reply)
  65. mean value of ct value (2 replies)
  66. Effect of EtBr on plasmid supercoiling (5 replies)
  67. fungi in antibody working solution (2 replies)
  68. Quantifying plasmid DNA (2 replies)
  69. How to know the exact plasmid size? (6 replies)
  70. Genomic DNA (9 replies)
  71. Viscous RNA samples (1 reply)
  72. Using Real Time PCR for Cell Viability (6 replies)
  73. 5-azadC vs 5-azacytidine (0 replies)
  74. Radio assay (0 replies)
  75. Tissue lysis. (1 reply)
  76. Any mRNA amplification kit for microarray? (0 replies)
  77. 5 azacytidine (0 replies)
  78. TGGE (1 reply)
  79. miniprep and maxiprep (9 replies)
  80. stable 3t3l1 transfection:serious help needed!!! (10 replies)
  81. bacterial strains (1 reply)
  82. a doubt about PCR gel purification (4 replies)
  83. funny patterns on a gel (4 replies)
  84. Promoter and genomic DNA (2 replies)
  85. PCR+enhancer (4 replies)
  86. DNA purification kit for Microinjection (1 reply)
  87. Vector Fill in for Blunt End Cloning (0 replies)
  88. Sequences of generally used plasmids? (7 replies)
  89. ORF FINDER (5 replies)
  90. transfcetion (1 reply)
  91. Program for mapping primers to gene - diagram maker, visualize (2 replies)
  92. Missed colonies grew to long! (2 replies)
  93. How to prevent RNA degradation in whole blood (3 replies)
  94. is there DNA ? (8 replies)
  95. methods to detect EGFP expression ?! (10 replies)
  96. Moved: Free journals subscribe freely (-- replies)
  97. Help in Liagtion Procedure (4 replies)
  98. stable transfectants (6 replies)
  99. Moved: Promoter sequence (-- replies)
  100. low melting point agarose (5 replies)
  101. plasmid copy number (2 replies)
  102. Co-transfection with PKJ-neo (evans et al), stable transfection (0 replies)
  103. Quantification of bacteria from digesta of chicken. (0 replies)
  104. RNA extraction (1 reply)
  105. PCR issue (2 replies)
  106. Purfiying Unmethylated Plasmid (3 replies)
  107. substractive hybridization (2 replies)
  108. Microarray chip (2 replies)
  109. Freeze thawing RNA (4 replies)
  110. Synthetic intron pIRES needed (2 replies)
  111. T4 DNA Ligase & Restriction Enzyme Expression (4 replies)
  112. PCR (1 reply)
  113. long PCR primer (7 replies)
  114. RNA appearance on 1.5% gel after DNase I digestion (2 replies)
  115. Ligation after SalI & XhoI Digestion (3 replies)
  116. Precipitation in NaOH on shelf (3 replies)
  117. mutation study (1 reply)
  118. size standard for TRFLP (0 replies)
  119. Random hexamer vs oligo dT vs gene specific primer for RT (2 replies)
  120. appearance RNA on 0.8% gel (0 replies)
  121. y pGEMT first then pGEX-2T (2 replies)
  122. No RNA detection (0 replies)
  123. how to remove a restricted endonuclease site (3 replies)
  124. Non-Radioactive In vitro Kinase Assay (2 replies)
  125. silica purification (1 reply)
  126. Shelf life of ampicillin in LB media? (6 replies)
  127. Pcr amplification (5 replies)
  128. Amplifying plasmid - non specific binding of primer (3 replies)
  129. sequencing problem after pcr (4 replies)
  130. DNA folding to form the loop (2 replies)
  131. smears on gel after PCR (1 reply)
  132. Gel run of pGEX-4T (6 replies)
  133. primer design (3 replies)
  134. DNA purification - long fragments wanted (1 reply)
  135. want gene knock out protocol (2 replies)
  136. Unidentified DNA bands (1 reply)
  137. Accidentally digested DNA overnight - still OK? (4 replies)
  138. SSCP (0 replies)
  139. Negative flouroscence in real time PCR (2 replies)
  140. Problems digesting PCR product (2 replies)
  141. decontamination (6 replies)
  142. Minimum number of PCR cycles to see a product? (6 replies)
  143. Make construct so it is only thing that can PCR after transfection (2 replies)
  144. Remove RNA after DNA mini-prep (1 reply)
  145. cloudy phase during RNA extraction with Trizol (10 replies)
  146. cDNA versus cRNA for a microarray (1 reply)
  147. Plasmid replication (13 replies)
  148. Troubleshoot ARMS PCR (1 reply)
  149. Transfection of vectors (8 replies)
  150. Capillary DNA Sequencing (6 replies)
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