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Molecular Biology
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Molecular Biology
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mRNA of bacteria
(1 reply)
DNA arrays for low gene expression ?
(1 reply)
PCR is6110 Tuberculosis
(2 replies)
MC57G cells and transfection
(1 reply)
Na2Hpo4 , NaH2po4 or both in Church buffer ?!
(2 replies)
Riboprobe northerns
(0 replies)
Why my CPM is higher after cDNA purification?
(0 replies)
Ostoblasts isolation
(0 replies)
electroelution
(3 replies)
electroelution
(1 reply)
pBABE Comp. Cells
(3 replies)
Trouble shooting RT-PCR
(3 replies)
Band in the negative control
(1 reply)
yeast complementation
(2 replies)
genomic library
(1 reply)
bacterial transformation with cell lysate
(0 replies)
ligation troubles...
(2 replies)
comparative genomics
(0 replies)
SYBR-Green amplification curve linear?
(0 replies)
transfection efficiency
(1 reply)
ldh-L promoter primers
(1 reply)
T4 Polymerase
(0 replies)
In situ hybridisations
(2 replies)
designing antisense oligo's...
(5 replies)
Radioactive cDNA probes
(0 replies)
Transformation and PCR questions
(2 replies)
Genomic DNA isolation
(2 replies)
RNA treatment
(2 replies)
Trouble in re-PCR Amplifying OSE products
(0 replies)
western blotting of expressed protein
(0 replies)
Fingerprinting
(0 replies)
facs of 2 fluorescens protein
(0 replies)
solubility of dna
(4 replies)
Experience with Real-time PCR platfoms
(0 replies)
how to HA tag a gene on the chromosome in E.coli?
(0 replies)
How to prepare double-stranded oligonucleotides?
(3 replies)
How to concentrate poly(A)+ mRNA
(2 replies)
cDNA library (in Lambda ZAP vector) screening
(2 replies)
fosmid
(0 replies)
PCR cloning
(2 replies)
BAC vector problems
(1 reply)
pCMV vector of stratagene
(0 replies)
RNA isolation
(2 replies)
3'DNA biotinylation kit
(0 replies)
detection of bacteria??
(1 reply)
about sequencing
(1 reply)
DNA extraction from formalinfixed tissue
(1 reply)
Electroporation cuvette 0.4 gap BIORAD
(0 replies)
need help in dnase!!!
(0 replies)
RNA smells like bleach
(2 replies)
protein-DNA binding
(1 reply)
Precipitation of DNA from CsCl samples
(1 reply)
analysis and purification for a [3H]labeled 14a.a
(0 replies)
How short can be the CpG island?
(3 replies)
plasmid with f1 origin - no expression
(0 replies)
LOH analysis
(0 replies)
Ligation into EGFP problems
(5 replies)
Promoterless Retroviral vector?
(1 reply)
Ligation with PLAFR
(1 reply)
PCR problem
(0 replies)
real-time PCR
(2 replies)
Which gene is the best internal control for RT-PCR
(4 replies)
how to determine end of gene?
(0 replies)
plasmid went missing
(5 replies)
Question about genomic base composition analysis
(2 replies)
How to identify plasmid
(2 replies)
Restriction Digestion
(4 replies)
Short DIG-RNA Probes
(0 replies)
Agarose gel extraction of 9-23 kb fragments
(0 replies)
Southern Blotting
(1 reply)
PCR
(2 replies)
Problem with PCR
(7 replies)
Problem in immunoprecipitation
(1 reply)
custom gene synthesis
(3 replies)
insert in the promoter region
(2 replies)
RNA from cultured Osteoblasts
(2 replies)
protein mistargeting
(1 reply)
Cellular HIV RNA vs Plasma HIV RNA
(0 replies)
RTpcR FOR hiv
(2 replies)
SSR fingerprinting
(0 replies)
cDNA from dsRNA
(1 reply)
Double mRNA/alcian blue detection
(1 reply)
Restriction sites
(3 replies)
Is there protein binding to any DNA
(0 replies)
SSC
(2 replies)
Knudsen Two-Hit Hypothesis?
(1 reply)
RNA extraction from snap frozen lung
(2 replies)
the precipitate on DEPC-H2O bottle
(1 reply)
T4 DNA ligase
(0 replies)
Need some background info!!!
(0 replies)
using CTAB for polysaccharide removal
(3 replies)
Phages
(1 reply)
BiPolar Clamps on PCR primers for TGGE
(1 reply)
need pME524 map
(0 replies)
in-competent cells
(1 reply)
western blot problem
(2 replies)
vector pFPV25.1
(0 replies)
isoelectric point
(0 replies)
Quickchange site directed mutagenesis
(2 replies)
microarray images
(1 reply)
RNA Extraction from ocular tissue
(2 replies)
RT-PCR;Northern-blotting
(4 replies)
Cosmid Vector
(0 replies)
Problems purifying DGGE bands
(8 replies)
Problems in my Multiplex PCR
(4 replies)
detection threshold of western blotting
(3 replies)
PCR product sequencing BD v3.1
(1 reply)
Plasmid pZB32 nodY::laz
(0 replies)
cloning upper stream of a gene
(1 reply)
GAPDH in serum
(2 replies)
colony growth question
(2 replies)
carrier tRNA
(2 replies)
HOW TO detect target gene
(5 replies)
how to get the flanking sequences
(3 replies)
Stock preparation of transformed L. lactis
(0 replies)
Primers: advice?
(1 reply)
small RNAs enrichment
(0 replies)
endothelial cell stable tranfection
(0 replies)
Shipping of plasmids and X-rays
(1 reply)
mRNA measurements-E.coli
(0 replies)
RNA and DNA extraction and PCR control
(1 reply)
transformation efficiency
(4 replies)
Largest viral insert?
(1 reply)
Minimal 35S Promoter
(0 replies)
RT-PCR work, western not, why??
(0 replies)
problem in invitro transcription/translation
(0 replies)
use Pen./Strep. in the media for trasnfection
(6 replies)
RNA Isolation
(5 replies)
southern blots
(1 reply)
utilisation of centricon filter tips(mllipore)
(0 replies)
monitoring gene silencing
(5 replies)
DNA-Protein Interaction in Yeast
(0 replies)
About Q-PCR(real-time PCR)
(1 reply)
transfection by viscosity method
(0 replies)
Errors in clones
(3 replies)
markers
(0 replies)
transfection
(2 replies)
fusion pcr
(1 reply)
full length cDNA extension
(0 replies)
Direct sequencing without cloning
(3 replies)
About the ABI fluorescent sensitive
(0 replies)
in vitro transcription
(0 replies)
in vitro transcription
(0 replies)
Calculation of Mutation Rates
(0 replies)
rat and human cells
(0 replies)
Sequencing PCR products
(2 replies)
Cloning using ds Oligos
(2 replies)
arrayCGH
(0 replies)
Has anyone used the DIG Chem-Link Labeling kit?
(0 replies)
arrayCGH software
(2 replies)
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