 Restriction enzymes |
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 Nov 2 2009, 08:03 AM
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#1
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member  Group: Members Posts: 4 Joined: 2-November 09 Member No.: 13694  |
HI
Is it true that by adding a few nucleotides to the restriction sites of some endonucleases you will increase its efficiency? I think I heard that in a lecture, but my Pi have never heard of it...so, I am confused now.... 
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Nov 2 2009, 04:38 PM
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#2
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 Veteran Group: Active Members Posts: 413 Joined: 26-January 09 From: Sydney, Australia Member No.: 6526 |
You add the extra bases to the end ofteh RE site ,to give hte enzyme something to hold onto. Check out the NEb website and see how many extra bases are required to improve your Re's efficiency.
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Nov 3 2009, 11:33 AM
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#3
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member Group: Members Posts: 4 Joined: 2-November 09 Member No.: 13694 |
Thanks!
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Nov 3 2009, 11:09 PM
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#4
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 Veteran Group: Active Members Posts: 747 Joined: 20-October 09 From: India!!! Member No.: 13357 |
QUOTE (swanny @ Nov 3 2009, 06:08 AM)  You add the extra bases to the end ofteh RE site ,to give hte enzyme something to hold onto. Check out the NEb website and see how many extra bases are required to improve your Re's efficiency. Tat was informative.. although i don work with RE's, this came as an interesting fact.. thanx for the thread canar -------------------- Support bacteria - They are the only culture some people have!!!
Cheers!!! |
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