Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

List All Videos


  Video Title Views Submission Date
Thumbnail

PCR Cloning


PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA
bioforum
Thumbnail

Pipetting Technique

bioforum
Thumbnail

Prepare hESCs for Cryopreservation

bioforum
Thumbnail

Proper Pipetting Techniques

bioforum
Thumbnail

Propidium Iodide Staining

bioforum
Thumbnail

Protein Quantification (Bradford Assay)

bioforum
Thumbnail

Pulling microinjection needles Part I

netron
Thumbnail

Pulling microinjection needles Part II

netron
Thumbnail

Real-time PCR

bioforum
Thumbnail

Real-time PCR Data Analysis with Excel

* * * * *
bioforum
Thumbnail

Real-Time PCR Thresholds and Where to Place Them

* * * * *
bioforum
Thumbnail

RNA Isolation and cDNA Synthesis

bioforum
Thumbnail

siRNA transfection


How to perform an siRNA transfection experiment using LipofectamineŽ RNAiMAX reagent.
bioforum
Thumbnail

siRNA transfection procedures

* * * * *
bioforum
Thumbnail

Subculture of hESCs by Collagenase IV

bioforum
Thumbnail

Subculture of hESCs by Mechanical Dissection

bioforum
Thumbnail

Transfection, Part I - Preparing the Mixure

bioforum
Thumbnail

Transfection, Part II

bioforum
Thumbnail

Using NCBI for RT-PCR Primer Design

* * * * *
intelligent selection of PCR primers. Topics include identifying transcript variants for your target of interest and selecting primers that amplify only specific transcripts. Adam also discusses the growing SNP database and the potential impact of SNPs o
bioforum
Thumbnail

Western Blot Visual Protocol: Phase 1: Sample Preparation

bioforum

Videos System v3.0.1 © 2019 DevFuse
Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.