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How to prepare neuronal cell cultures

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DNA Barcoding Protocol: Isolating DNA

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Arabidopsis Seed Planting Protocol

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Agarose DNA Gel Preparation

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Alkaline Phosphatase Stain

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Subculture of hESCs by Mechanical Dissection

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Subculture of hESCs by Collagenase IV

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Prepare hESCs for Cryopreservation

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EB formation

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H&E Staining

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Mycoplasma Detection - PCR Method

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Real-time PCR

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PCR Cloning


PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. PCR cloning procedure consisting of the four following steps: (1) production of a fragment of the gene using PCR, (2) digestion of genomic DNA
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Using NCBI for RT-PCR Primer Design

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intelligent selection of PCR primers. Topics include identifying transcript variants for your target of interest and selecting primers that amplify only specific transcripts. Adam also discusses the growing SNP database and the potential impact of SNPs o
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siRNA transfection procedures

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siRNA transfection


How to perform an siRNA transfection experiment using LipofectamineŽ RNAiMAX reagent.
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Transfection, Part II

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Transfection, Part I - Preparing the Mixure

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Bacterial Transformation

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DNA Transformation into Bacteria

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