Phenol chloroform extraction of DNA (View forum version)



Nilafhiosagam

Posted 08 April 2009 - 05:07 AM

I'm writing up a lab report and I was wondering if anybody had a link to a good explanation to the theory behind a phenol chloroform extraction of DNA?

Thanks

bob1

Posted 08 April 2009 - 04:49 PM

Not a link, but try finding a copy of Sambrook, Fritsch and Maniatis, Molecular Cloning: A Laboratory Manual.

HomeBrew

Posted 08 April 2009 - 05:52 PM

Different solubilities. Nucleic acids are more soluble in water, and thus predominate in the aqueous phase, whereas proteins prefer an organic solvent, and thus separate to the phenolic phase.

Nilafhiosagam

Posted 09 April 2009 - 07:51 AM

Thank you for the replies.