differentiation of sh-sy5y (View forum version)



Snoopy

Posted 15 March 2009 - 06:25 AM

Hello,
I try to differentiate sh-sy5y neuroblastoma cells with 10 microM without that much success.

I want to know at which density you seed them for differentiation 'couse i use 20000/cm2 and it might be too much.

what else could i do to make them be more differentiated.

Waiting for your answer

snoopy :D

labrat612

Posted 15 March 2009 - 11:15 AM

What agent were you using to differentiate? I've typically used retinoic acid.

Snoopy

Posted 16 March 2009 - 01:59 AM

I use 10 micromolar of retinoic acid with retinoic acid in full medium (10% Fetal calf serum)

scolix

Posted 21 March 2009 - 10:56 PM

Hello,
I try to differentiate sh-sy5y neuroblastoma cells with 10 microM without that much success.

I want to know at which density you seed them for differentiation 'couse i use 20000/cm2 and it might be too much.

what else could i do to make them be more differentiated.

Waiting for your answer

snoopy :D



try to add some neurotrophic factors like NGF or GDNF to promote neurite differentiation.