DNA shearing with Misonix 4000 and microplates cup horn (View forum version)



Isazzurra

Posted 11 March 2009 - 09:50 AM

Hi everybody,
I'm going crazy trying to shear the DNA of my Saos2 and MCF-7 with a Misonix 4000 sonicator with a microplate cup horn.
Any suggestion?
Please!!

pcrman

Posted 15 March 2009 - 10:02 AM

What is your propblem? unable to shear DNA? what conditions are you using. Without any of these info, it is hard to troubleshoot.

tjmuwyj

Posted 15 March 2009 - 11:28 PM

there is a girl also doing DNA shearing with sonication. maybe you can have a communication.

but I want to know how to see or download the attatchment in the following site:

http://www.protocol-...14943more2.html