Dissolving solid reagent in DMSO (View forum version)



kusuma

Posted 18 April 2015 - 02:17 AM

Hi all,

 

I have issued that remain unsolved till today.

I want to make 200 µL of 10 mM 2-Nitrobenzoic aldehyde (2 NBA) which dissolved in dimethylsulfoxide [DMSO].

I know that first I need to make 10 mM NBA then I dissolved this to DMSO.

But how many volume of 2 NBA I need to used? Since NBA present in solid form.

Highly appreciate for your helps.

 

Note:

Mw of 2 NBA 151.12

Mw of DMSO 78.13

 

Thanks.

 

Kus


bob1

Posted 19 April 2015 - 01:45 AM

Use mass= number of moles x molar mass and number of moles = volume x concentration.

 

Remember that mM is millimole per litre and molar masses are in grams per mole!!!