Sonication: why is foam bad? (View forum version)



TurtleBob

Posted 10 October 2014 - 12:45 PM

Hi All, 

 

I see lots of tips on "ways to avoid foam", and that "foam is bad". But I can't seem to find what's bad about it. Why do you want to avoid foam during sonication?

 

Also, if it's true that water bath sonicators don't produce foam, why aren't they the alternative?

Is there a difference in how bath and probe sonicators disrupt tissue? 

 

 

Many thanks for any help!

 

TurtleBob


bob1

Posted 12 October 2014 - 01:54 PM

Foam is bad as it increases the surface area of the solution and thereby causes oxidation of the proteins and hence degradation. It also causes strong forces that can pull complexes apart.

 

Bath sonicators are usually too gentle to do what you want a sonicator to do, which is why people tend to go for a probe sonicator.


TurtleBob

Posted 12 October 2014 - 05:44 PM

Foam is bad as it increases the surface area of the solution and thereby causes oxidation of the proteins and hence degradation. It also causes strong forces that can pull complexes apart.

 

Bath sonicators are usually too gentle to do what you want a sonicator to do, which is why people tend to go for a probe sonicator.

thank you!