Can serum free medium inactivate trypsin? (View forum version)



arjuna

Posted 03 December 2013 - 06:12 PM

hi, i am first time in forum,

my concern is,

can serum free medium inactivate trypsin?? i didnot have this idea so mistakenly i used serum free medium to inactivate trypsin which resulted transfected cells(gfp +ve) lost gfp(confirmed by florescence microscope) but still cells are alive. can anyone help pls.


bob1

Posted 04 December 2013 - 02:56 AM

Short answer, no.  Long answer: maybe, but it will depend on the additives in the medium.