Rewetting the dry PVDF after secondary antibody with MetOH or not? (View forum version)



Josip Madunic

Posted 23 September 2013 - 01:39 AM

I have been exposing the PVDF membrane with film during the weekend, and now my membranes have dried out in Saran wrap. I would like to repeat secondary antibody step (same antibody, without any stripping), but I am not sure if I should rewet the membrane (which now has stable primary-secondary-HRP complex) with MetOH before washing it in TBST and incubating in new secondary antibody?

 

Will the MetOH somehow disrupt primary-secondary-HRP complex on the membrane?


theo22

Posted 23 September 2013 - 02:56 AM

I would just soak it in TBST. I am used to store my used membranes in the freezer and just soak them in TBST before adding another antibody. That works very well.